NBCe2 Polyklonaler Antikörper

NBCe2 Polyklonal Antikörper für IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 26150-1-PBS

Synonyme

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  IHC staining of human liver cancer using 26150-1-AP (same clone as 26150-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 26150-1-AP (NBCe2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26150-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 26150-1-AP (same clone as 26150-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 26150-1-AP (NBCe2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26150-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse kidney using 26150-1-AP (same clone as 26150-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 26150-1-AP (NBCe2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26150-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 26150-1-AP (same clone as 26150-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 26150-1-AP (NBCe2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26150-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 26150-1-AP (same clone as 26150-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using NBCe2 antibody (26150-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 26150-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

26150-1-PBS bindet in IHC, IF/ICC, Indirect ELISA NBCe2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NBCe2 fusion protein Ag23558
• Vollständiger Name: solute carrier family 4, sodium bicarbonate cotransporter, member 5
• Beobachtetes Molekulargewicht: 108-127 kDa
• GenBank-Zugangsnummer: BC109221
• Gene symbol: SLC4A5
• Gene ID (NCBI): 57835
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NBCe2, also named as SLC4A5 and NBC4, belongs to the anion exchanger (TC 2.A.31) family. NBCe2 mediates electrogenic Na+:HCO3 − transport, and can operate in both a 1:2, and 1:3 manner, but the transport stoichiometry as well as directionality have not been defined for the renal NBCe2. The expression of NBCe2 has been reported in nearly all kidney nephron segments and CDs, indicating that some species-specificity might be present (PMID: 32547422). NBCe2 has 8 isoforms with the molecular mass of 108-127 kDa.