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NBN / NBS1 Rekombinanter Antikörper

NBN / NBS1 Rekombinant Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1C16

Kat-Nr. : 82884-1-PBS

Synonyme

AT V1, AT V2, ATV, FLJ10155, NBN, NBS, NBS1, nibrin, P95

Filter:
  • Western Blot (WB) analysis of various lysates using NBN / NBS1 Recombinant antibody (82884-1-RR)

    WB analysis using 82884-1-RR (same clone as 82884-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82884-1-RR (NBN / NBS1 antibody) at dilution of 1:5100 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82884-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

82884-1-PBS bindet in WB, Indirect ELISA NBN / NBS1 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen Peptid
Vollständiger Name nibrin
Berechnetes Molekulargewicht 85 kDa
Beobachtetes Molekulargewicht 90-95 kDa
GenBank-ZugangsnummerBC136803
Gene symbol NBN
Gene ID (NCBI) 4683
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NBN, also named as NBS, NBS1, and P95, is a component of the MRE11/RAD50/NBN (MRN complex) which plays a critical role in the cellular response to DNA damage and the maintenance of chromosome integrity. The complex is involved in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity, cell cycle checkpoint control, and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11A. NBN modulates the DNA damage signal sensing by recruiting PI3/PI4-kinase family members ATM, ATR, and probably DNA-PKcs to the DNA damage sites and activating their functions. NBN also functions in telomere length maintenance by generating the 3' overhang which serves as a primer for telomerase-dependent telomere elongation. NBN is a major player in the control of intra-S-phase checkpoints and there is some evidence that NBN is involved in G1 and G2 checkpoints. Defects in NBN are the cause of Nijmegen breakage syndrome (NBS). Defects in NBN are a cause of genetic susceptibility to breast cancer (BC). Defects in NBN may be associated with aplastic anemia. Defects in NBN might play a role in the pathogenesis of childhood acute lymphoblastic leukemia (ALL). The antibody is specific to NBN. The full-length NBN protein, with an apparent molecular weight of 95 kDa and the two protein fragments of 26 and 70 kDa arising from the c.657_661del5 (p.K219fsX19) mutation, and the 80 kDa protein found in patient RR with the mutation c.742_743insGG leading to excision of exons 6 and 7 from the NBN mRNA are shown. (PMID: 26265251) The predicted molecular weight of NBN protein (p95) is 85kDa, and actually detection result is about 95kDa(PMID: 23762398).