NBR1 Monoklonaler Antikörper

NBR1 Monoklonal Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1F2B2

Kat-Nr. : 68062-1-PBS

Synonyme

1A1 3B, 1A13B, 1F2B2, Cell migration-inducing gene 19 protein, FLJ55359

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  WB analysis using 68062-1-Ig (same clone as 68062-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68062-1-Ig (NBR1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68062-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 68062-1-Ig (same clone as 68062-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using NBR1 antibody (68062-1-Ig, Clone: 1F2B2 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68062-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

68062-1-PBS bindet in WB, IF/ICC, Indirect ELISA NBR1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: NBR1 fusion protein Ag8652
• Vollständiger Name: neighbor of BRCA1 gene 1
• Berechnetes Molekulargewicht: 966 aa, 107 kDa
• Beobachtetes Molekulargewicht: 140 kDa
• GenBank-Zugangsnummer: BC009808
• Gene symbol: NBR1
• Gene ID (NCBI): 4077
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NBR1, also named as 1A13B, KIAA0049 and M17S2, acts probably as a receptor for selective autophagosomal degradation of ubiquitinated targets. NBR1 and P62 can bind to autophagic effector proteins (Atg8 in yeast, MAP1LC3 protein family in mammals) anchored in the membrane of autophagosomes. It is a highly conserved multidomain scaffold protein with proposed roles in endocytic trafficking and selective autophagy. NBR1 is a novel PB1 adapter in Th2 differentiation and asthma. It functions as an autophagy receptor involved in targeting ubiquitinated proteins for degradation. It also has a dual role as a scaffold protein to regulate growth-factor receptor and downstream signaling pathways. Observed MW of NBR1 is 140 kDa (PMID: 22654911, PMID: 22484440).