NCALD Monoklonaler Antikörper

NCALD Monoklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

6B4E10

Kat-Nr. : 66088-1-PBS

Synonyme

NCALD, neurocalcin delta, neurocalcin δ

---

• 

  WB analysis using 66088-1-Ig (same clone as 66088-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66088-1-Ig (NCALD antibody) at dilution of 1:1600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66088-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66088-1-Ig (same clone as 66088-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66088-1-Ig (NCALD antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66088-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human brain using 66088-1-Ig (same clone as 66088-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 66088-1-Ig (NCALD antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66088-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66088-1-Ig (same clone as 66088-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66088-1-Ig (NCALD antibody) at dilution of 1:1600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66088-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human cerebellum using 66088-1-Ig (same clone as 66088-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cerebellum using 66088-1-Ig(NCALD antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66088-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human testis using 66088-1-Ig (same clone as 66088-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis slide using 66088-1-Ig (NCALD Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 66088-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human testis using 66088-1-Ig (same clone as 66088-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis slide using 66088-1-Ig (NCALD Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 66088-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 66088-1-Ig (same clone as 66088-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66088-1-Ig (NCALD antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66088-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 66088-1-Ig (same clone as 66088-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66088-1-Ig (NCALD antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66088-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse cerebellum using 66088-1-Ig (same clone as 66088-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 66088-1-Ig (NCALD antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66088-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse cerebellum using 66088-1-Ig (same clone as 66088-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 66088-1-Ig (NCALD antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66088-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse cerebellum using 66088-1-Ig (same clone as 66088-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 66088-1-Ig (NCALD antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66088-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse cerebellum using 66088-1-Ig (same clone as 66088-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 66088-1-Ig (NCALD antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66088-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human cerebellum using 66088-1-Ig (same clone as 66088-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cerebellum using 66088-1-Ig(NCALD antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66088-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 66088-1-Ig (same clone as 66088-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 66088-1-Ig (NCALD antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66088-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

66088-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA NCALD und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: NCALD fusion protein Ag18760
• Vollständiger Name: neurocalcin delta
• Berechnetes Molekulargewicht: 193 aa, 22 kDa
• Beobachtetes Molekulargewicht: 22 kDa
• GenBank-Zugangsnummer: BC036098
• Gene symbol: NCALD
• Gene ID (NCBI): 83988
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NCALD(Neurocalcin-delta) is a member of the EF-hand calcium-binding protein superfamily.It may be involved in the calcium-dependent regulation of rhodopsin phosphorylation.It is a high-affinity Ca2+ sensor (Kd of approx. 0.6 uM), which is most abundantly expressed in the Purkinje cells of the cerebellum(PMID:11964161).This antibody is specific to NCALD.