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NCAM1/CD56 Polyklonaler Antikörper
NCAM1/CD56 Polyklonal Antikörper für FC, IHC, WB, ELISA
Wirt / Isotyp
Kaninchen / IgG
Getestete Reaktivität
Hausschwein, human, Maus, Ratte
Anwendung
WB, IHC, IF, FC, ELISA
Konjugation
Unkonjugiert
Kat-Nr. : 14255-1-AP
Synonyme
Galerie der Validierungsdaten
Geprüfte Anwendungen
| Erfolgreiche Detektion in WB | Maushirngewebe, Hausschwein-Hirngewebe, Maus-Cerebellum-Gewebe, Rattenhirngewebe |
| Erfolgreiche Detektion in IHC | humanes Lungenkarzinomgewebe, humanes Appendizitis-Gewebe, humanes Tonsillitisgewebe, Insulinomgewebe, Maushirngewebe, Rattenhirngewebe Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen. |
| Erfolgreiche Detektion in FC | SH-SY5Y-Zellen |
Empfohlene Verdünnung
| Anwendung | Verdünnung |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunhistochemie (IHC) | IHC : 1:2000-1:20000 |
| Durchflusszytometrie (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Veröffentlichte Anwendungen
| KD/KO | See 3 publications below |
| WB | See 13 publications below |
| IHC | See 23 publications below |
| IF | See 20 publications below |
Produktinformation
14255-1-AP bindet in WB, IHC, IF, FC, ELISA NCAM1/CD56 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten
| Getestete Reaktivität | Hausschwein, human, Maus, Ratte |
| In Publikationen genannte Reaktivität | human, Hausschwein, Maus, Ratte |
| Wirt / Isotyp | Kaninchen / IgG |
| Klonalität | Polyklonal |
| Typ | Antikörper |
| Immunogen | NCAM1/CD56 fusion protein Ag5528 |
| Vollständiger Name | neural cell adhesion molecule 1 |
| Berechnetes Molekulargewicht | 95 kDa |
| Beobachtetes Molekulargewicht | 120 kDa, 140 kDa, 180 kDa |
| GenBank-Zugangsnummer | BC047244 |
| Gene symbol | NCAM1 |
| Gene ID (NCBI) | 4684 |
| Konjugation | Unkonjugiert |
| Form | Liquid |
| Reinigungsmethode | Antigen-Affinitätsreinigung |
| Lagerungspuffer | PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3. |
| Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
Neural cell adhesion molecule 1 (NCAM1, also known as CD56) is a cell adhesion glycoprotein of the immunoglobulin (Ig) superfamily. It is a multifunction protein involved in synaptic plasticity, neurodevelopment, and neurogenesis. NCAM1 is expressed on human neurons, glial cells, skeletal muscle cells, NK cells and a subset of T cells, and the expression is observed in a wide variety of human tumors, including myeloma, myeloid leukemia, neuroendocrine tumors, Wilms' tumor, neuroblastoma, and NK/T cell lymphomas. Three major isoforms of NCAM1, with molecular masses of 120, 140, and 180 kDa, are generated by alternative splicing of mRNA (PMID: 9696812). The glycosylphosphatidylinositol (GPI)-anchored NCAM120 and the transmembrane NCAM140 and NCAM180 consist of five Ig-like domains and two fibronection-type III repeats (FNIII). All three forms can be posttranslationally modified by addition of polysialic acid (PSA) (PMID: 14976519). Several other isofroms have also been described (PMID: 1856291).
Protokolle
| Produktspezifische Protokolle | |
|---|---|
| WB protocol for NCAM1/CD56 antibody 14255-1-AP | Protokoll herunterladen |
| IHC protocol for NCAM1/CD56 antibody 14255-1-AP | Protokoll herunterladen |
| FC protocol for NCAM1/CD56 antibody 14255-1-AP | Protokoll herunterladen |
| Standard-Protokolle | |
|---|---|
| Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
| Species | Application | Title |
|---|---|---|
Nat Med Selective modulation of the androgen receptor AF2 domain rescues degeneration in spinal bulbar muscular atrophy. | ||
Cell Metab Multiplexed In Situ Imaging Mass Cytometry Analysis of the Human Endocrine Pancreas and Immune System in Type 1 Diabetes. | ||
Sci Adv Gene therapy with AR isoform 2 rescues spinal and bulbar muscular atrophy phenotype by modulating AR transcriptional activity. | ||
J Clin Invest Thioredoxin activity confers resistance against oxidative stress in tumor-infiltrating NK cells. | ||
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Kenzo (Verified Customer) (01-09-2023) | Worked great. The staining pattern was consistent with what has been reported.
|
FH Emma (Verified Customer) (11-29-2021) | Works well by IF on FFPE tissue @ 1:1000. We used a Tris-EDTA antigen retrieval.
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FH Toni (Verified Customer) (02-28-2019) | IP conditions:1ug of rbt anti-NCAM1 was added to 100ug human cortex homogenate (0.5ug/ul) in Tris-sucrose buffer with 1% SDS and 1% Triton X100 thenincubated rotating ON at 4C. Sample was then added to 50ul TBST-washed sheep anti-rabbit magnetic beads and incubated rotating 3hr at 4C. Following 4 TBST washes, bound proteins were eluted with 30ul of elution buffer containing SDS and BME. WB conditions:Samples subjected to SDS-PAGE on 4-12% Bis-Tris gels followed by semi-dry transfer to nitrocellulose membranes using standard conditions. Membranes were blocked for 1hr at RT in 50% LiCor Odyssey blocking buffer (TBS) then probed with either 1:5000 (v/v) rabbit anti-NCAM1 (Proteintech) or 1:1000 (v/v) goat anti-NCAM1 (R&D Systems) in 50% LiCor Odyssey blocking buffer (TBS + 0.05% Tween-20) ON at 4C. Following TBS+ 0.1% Tween-20 washes, membranes were incubated with the appropriate IR-dye labeled secondary antibody for 1hr at RT, TBST washed, then scanned.*you have my permission to edit the comments above and crop, but not alter, the image provided if you wish to remove reference to an antibody from another company.
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