NCAM1/CD56 Rekombinanter Antikörper

• Featured Product
• KD/KO Validated

NCAM1/CD56 Rekombinant Antikörper für WB, IHC, IF-P, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, IF-P, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

CloneNo.

6N11

Kat-Nr. : 82715-1-RR

Synonyme

CD56, NCAM1, 6N11, MSK39, N CAM 1

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  WB analysis of U-251 using 82715-1-RR

  WB result of NCAM1/CD56 antibody (82715-1-RR; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NCAM1/CD56 transfected U-251 cells.

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  WB analysis using 82715-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82715-1-RR (NCAM1/CD56 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 82715-1-RR

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 82715-1-RR (NCAM1/CD56 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of U-251 using 82715-1-RR

  U-251 cells were subjected to SDS PAGE followed by western blot with 82715-1-RR (NCAM1/CD56 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 82715-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82715-1-RR (NCAM1/CD56 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 82715-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82715-1-RR (NCAM1/CD56 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse colon using 82715-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 82715-1-RR (NCAM1/CD56 antibody) at dilution of 1:8000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 82715-1-RR

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 82715-1-RR (NCAM1/CD56 antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse brain using 82715-1-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using NCAM1/CD56 antibody (82715-1-RR, Clone: 6N11 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 82715-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82715-1-RR against Human NCAM1 were performed. The affinity constant is 0.23 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Hausschwein-Hirngewebe, C6-Zellen, Maushirngewebe, Neuro-2a-Zellen, Rattenhirngewebe, SH-SY5Y-Zellen, U-251-Zellen
• Erfolgreiche Detektion in IHC: Maushirngewebe, humanes Tonsillitisgewebe, Maus-Kolongewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Maushirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:200-1:1000
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IHC: See 1 publications below

Produktinformation

82715-1-RR bindet in WB, IHC, IF-P, ELISA NCAM1/CD56 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: NCAM1/CD56 fusion protein Ag5528
• Vollständiger Name: neural cell adhesion molecule 1
• Berechnetes Molekulargewicht: 95 kDa
• Beobachtetes Molekulargewicht: 120 kDa, 140 kDa, 180 kDa
• GenBank-Zugangsnummer: BC047244
• Gene symbol: NCAM1
• Gene ID (NCBI): 4684
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Neural cell adhesion molecule 1 (NCAM1, also known as CD56) is a cell adhesion glycoprotein of the immunoglobulin (Ig) superfamily. It is a multifunction protein involved in synaptic plasticity, neurodevelopment, and neurogenesis. NCAM1 is expressed on human neurons, glial cells, skeletal muscle cells, NK cells and a subset of T cells, and the expression is observed in a wide variety of human tumors, including myeloma, myeloid leukemia, neuroendocrine tumors, Wilms' tumor, neuroblastoma, and NK/T cell lymphomas. Three major isoforms of NCAM1, with molecular masses of 120, 140, and 180 kDa, are generated by alternative splicing of mRNA (PMID: 9696812). The glycosylphosphatidylinositol (GPI)-anchored NCAM120 and the transmembrane NCAM140 and NCAM180 consist of five Ig-like domains and two fibronection-type III repeats (FNIII). All three forms can be posttranslationally modified by addition of polysialic acid (PSA) (PMID: 14976519). Several other isofroms have also been described (PMID: 1856291).

Publikationen

Species	Application	Title
human	IHC	Mol Med; Crosstalk between cancer-associated fibroblasts and non-neuroendocrine tumor cells in small cell lung cancer involves in glycolysis and antigen-presenting features; Authors - Yuanhua Lu