NCAM1/CD56 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

NCAM1/CD56 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 14255-1-PBS

Synonyme

CD56, NCAM1, MSK39, N CAM 1, NCAM

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  WB analysis of Neuro-2a using 14255-1-AP (same clone as 14255-1-PBS)

  WB result of NCAM1/CD56 antibody (14255-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NCAM1/CD56 transfected Neuro-2a cells. This data was developed using the same antibody clone with 14255-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain using 14255-1-AP (same clone as 14255-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14255-1-AP (NCAM1/CD56 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14255-1-PBS in a different storage buffer formulation.

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  WB analysis using 14255-1-AP (same clone as 14255-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 14255-1-AP (NCAM1/CD56 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14255-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 14255-1-AP (same clone as 14255-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 14255-1-AP (NCAM1/CD56 antibody) at dilution of 1:16000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14255-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 14255-1-AP (same clone as 14255-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 14255-1-AP (NCAM1/CD56 antibody) at dilution of 1:16000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14255-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse colon using 14255-1-AP (same clone as 14255-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 14255-1-AP (NCAM1/CD56 antibody) at dilution of 1:16000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14255-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse colon using 14255-1-AP (same clone as 14255-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 14255-1-AP (NCAM1/CD56 antibody) at dilution of 1:16000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14255-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 14255-1-AP (same clone as 14255-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human small cell lung carcinoma tissue slide using 14255-1-AP (NCAM1/CD56 antibody) at dilution of 1:16000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14255-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 14255-1-AP (same clone as 14255-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human small cell lung carcinoma slide using 14255-1-AP (NCAM1/CD56 antibody) at dilution of 1:16000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14255-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 14255-1-AP (same clone as 14255-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 14255-1-AP (NCAM1/CD56 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14255-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 14255-1-AP (same clone as 14255-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 14255-1-AP (NCAM1/CD56 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14255-1-PBS in a different storage buffer formulation.

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  IHC staining of Insulinoma using 14255-1-AP (same clone as 14255-1-PBS)

  Immunohistochemical analysis of paraffin-embedded Insulinoma tissue slide using 14255-1-AP (NCAM1/CD56 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14255-1-PBS in a different storage buffer formulation.

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  IHC staining of Insulinoma using 14255-1-AP (same clone as 14255-1-PBS)

  Immunohistochemical analysis of paraffin-embedded Insulinoma tissue slide using 14255-1-AP (NCAM1/CD56 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14255-1-PBS in a different storage buffer formulation.

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  IHC staining of human appendicitis using 14255-1-AP (same clone as 14255-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 14255-1-AP (NCAM1/CD56 antibody) at dilution of 1:16000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14255-1-PBS in a different storage buffer formulation.

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  IHC staining of human appendicitis using 14255-1-AP (same clone as 14255-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 14255-1-AP (NCAM1/CD56 antibody) at dilution of 1:16000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14255-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 14255-1-AP (same clone as 14255-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 14255-1-AP (NCAM1/CD56 antibody) at dilution of 1:64000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14255-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 14255-1-AP (same clone as 14255-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 14255-1-AP (NCAM1/CD56 antibody) at dilution of 1:64000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14255-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 14255-1-AP (same clone as 14255-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14255-1-AP (NCAM1/CD56 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14255-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 14255-1-AP (same clone as 14255-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14255-1-AP (NCAM1/CD56 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14255-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat brain using 14255-1-AP (same clone as 14255-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 14255-1-AP (NCAM1/CD56 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14255-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat brain using 14255-1-AP (same clone as 14255-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 14255-1-AP (NCAM1/CD56 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14255-1-PBS in a different storage buffer formulation.

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  IF Staining of SH-SY5Y using 14255-1-AP (same clone as 14255-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using NCAM1/CD56 antibody (14255-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 14255-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

14255-1-PBS bindet in WB, IHC, IF/ICC, ELISA NCAM1/CD56 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NCAM1/CD56 fusion protein Ag5528
• Vollständiger Name: neural cell adhesion molecule 1
• Berechnetes Molekulargewicht: 95 kDa
• Beobachtetes Molekulargewicht: 120 kDa, 140 kDa, 180 kDa
• GenBank-Zugangsnummer: BC047244
• Gene symbol: NCAM1
• Gene ID (NCBI): 4684
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Neural cell adhesion molecule 1 (NCAM1, also known as CD56) is a cell adhesion glycoprotein of the immunoglobulin (Ig) superfamily. It is a multifunction protein involved in synaptic plasticity, neurodevelopment, and neurogenesis. NCAM1 is expressed on human neurons, glial cells, skeletal muscle cells, NK cells and a subset of T cells, and the expression is observed in a wide variety of human tumors, including myeloma, myeloid leukemia, neuroendocrine tumors, Wilms' tumor, neuroblastoma, and NK/T cell lymphomas. Three major isoforms of NCAM1, with molecular masses of 120, 140, and 180 kDa, are generated by alternative splicing of mRNA (PMID: 9696812). The glycosylphosphatidylinositol (GPI)-anchored NCAM120 and the transmembrane NCAM140 and NCAM180 consist of five Ig-like domains and two fibronection-type III repeats (FNIII). All three forms can be posttranslationally modified by addition of polysialic acid (PSA) (PMID: 14976519). Several other isofroms have also been described (PMID: 1856291).