NCAM1/CD56 Rekombinanter Antikörper

• Featured Product
• KD/KO Validated

NCAM1/CD56 Rekombinant Antikörper für WB, IHC, IF-P, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, IF-P, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

6N11

Kat-Nr. : 82715-1-PBS

Synonyme

CD56, NCAM1, 6N11, MSK39, N CAM 1

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  WB analysis of U-251 using 82715-1-RR (same clone as 82715-1-PBS)

  WB result of NCAM1/CD56 antibody (82715-1-RR; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NCAM1/CD56 transfected U-251 cells. This data was developed using the same antibody clone with 82715-1-PBS in a different storage buffer formulation.

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  WB analysis using 82715-1-RR (same clone as 82715-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82715-1-RR (NCAM1/CD56 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82715-1-PBS in a different storage buffer formulation.

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  WB analysis of SH-SY5Y using 82715-1-RR (same clone as 82715-1-PBS)

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 82715-1-RR (NCAM1/CD56 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82715-1-PBS in a different storage buffer formulation.

• 

  WB analysis of U-251 using 82715-1-RR (same clone as 82715-1-PBS)

  U-251 cells were subjected to SDS PAGE followed by western blot with 82715-1-RR (NCAM1/CD56 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82715-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 82715-1-RR (same clone as 82715-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82715-1-RR (NCAM1/CD56 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82715-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 82715-1-RR (same clone as 82715-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82715-1-RR (NCAM1/CD56 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82715-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse colon using 82715-1-RR (same clone as 82715-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 82715-1-RR (NCAM1/CD56 antibody) at dilution of 1:8000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82715-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 82715-1-RR (same clone as 82715-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 82715-1-RR (NCAM1/CD56 antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82715-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse brain using 82715-1-RR (same clone as 82715-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using NCAM1/CD56 antibody (82715-1-RR, Clone: 6N11 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82715-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 82715-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82715-1-RR against Human NCAM1 were performed. The affinity constant is 0.23 nM.

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Geprüfte Anwendungen

Produktinformation

82715-1-PBS bindet in WB, IHC, IF-P, Indirect ELISA NCAM1/CD56 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: NCAM1/CD56 fusion protein Ag5528
• Vollständiger Name: neural cell adhesion molecule 1
• Berechnetes Molekulargewicht: 95 kDa
• Beobachtetes Molekulargewicht: 120 kDa, 140 kDa, 180 kDa
• GenBank-Zugangsnummer: BC047244
• Gene symbol: NCAM1
• Gene ID (NCBI): 4684
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Neural cell adhesion molecule 1 (NCAM1, also known as CD56) is a cell adhesion glycoprotein of the immunoglobulin (Ig) superfamily. It is a multifunction protein involved in synaptic plasticity, neurodevelopment, and neurogenesis. NCAM1 is expressed on human neurons, glial cells, skeletal muscle cells, NK cells and a subset of T cells, and the expression is observed in a wide variety of human tumors, including myeloma, myeloid leukemia, neuroendocrine tumors, Wilms' tumor, neuroblastoma, and NK/T cell lymphomas. Three major isoforms of NCAM1, with molecular masses of 120, 140, and 180 kDa, are generated by alternative splicing of mRNA (PMID: 9696812). The glycosylphosphatidylinositol (GPI)-anchored NCAM120 and the transmembrane NCAM140 and NCAM180 consist of five Ig-like domains and two fibronection-type III repeats (FNIII). All three forms can be posttranslationally modified by addition of polysialic acid (PSA) (PMID: 14976519). Several other isofroms have also been described (PMID: 1856291).