NCAM2 Polyklonaler Antikörper

NCAM2 Polyklonal Antikörper für WB, IHC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 13850-1-PBS

Synonyme

N CAM 2, NCAM 2, NCAM-2, NCAM21

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  WB analysis using 13850-1-AP (same clone as 13850-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 13850-1-AP (NCAM2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13850-1-PBS in a different storage buffer formulation.

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  WB analysis of human brain using 13850-1-AP (same clone as 13850-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 13850-1-AP (NCAM2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13850-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse brain using 13850-1-AP (same clone as 13850-1-PBS)

  IP result of anti-NCAM2 (IP:13850-1-AP, 4ug; Detection:13850-1-AP 1:5000) with mouse brain tissue lysate 2100 ug. This data was developed using the same antibody clone with 13850-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 13850-1-AP (same clone as 13850-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13850-1-AP (NCAM2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13850-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 13850-1-AP (same clone as 13850-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13850-1-AP (NCAM2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13850-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 13850-1-AP (same clone as 13850-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13850-1-AP (NCAM2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13850-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human gliomas using 13850-1-AP (same clone as 13850-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13850-1-AP (NCAM2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13850-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

13850-1-PBS bindet in WB, IHC, IP, Indirect ELISA NCAM2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NCAM2 fusion protein Ag4821
• Vollständiger Name: neural cell adhesion molecule 2
• Berechnetes Molekulargewicht: 93 kDa
• Beobachtetes Molekulargewicht: 100-125 kDa
• GenBank-Zugangsnummer: BC052946
• Gene symbol: NCAM2
• Gene ID (NCBI): 4685
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The mammalian NCAM family has two members, NCAM1 and NCAM2 (PMID: 9729879; 20439718). Neural cell adhesion molecule 2 (NCAM2, also known as NCAM21) is a single-pass type I membrane protein that is highly expressed in the brain, which may play important roles in selective fasciculation and zone-to-zone projection of the primary olfactory axons or in neurodegenerative diseases such as Alzheimer's disease (PMID: 26611261). NCAM2 has two isoforms: NCAM2.1 bearing transmembrane and cytoplasmic domains and the shorter NCAM2.2 isoform, which is GPI-anchored (PMID: 9334170; 9221781). The molecular weight of NCAM2 is around 125 kDa and 93 kDa (PMID: 9334170; 3171685).