NCAPD2 Polyklonaler Antikörper

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• KD/KO Validated

NCAPD2 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 13382-1-PBS

Synonyme

Condensin complex subunit 1, CNAP1, Chromosome-associated protein D2, Chromosome condensation-related SMC-associated protein 1, CAPD2

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  WB analysis using 13382-1-AP (same clone as 13382-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 13382-1-AP (NCAPD2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13382-1-PBS in a different storage buffer formulation.

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  WB analysis of COLO 320 using 13382-1-AP (same clone as 13382-1-PBS)

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 13382-1-AP (NCAPD2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13382-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 13382-1-AP (same clone as 13382-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 13382-1-AP (NCAPD2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13382-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 13382-1-AP (same clone as 13382-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 13382-1-AP (NCAPD2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13382-1-PBS in a different storage buffer formulation.

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  WB analysis of K-562 using 13382-1-AP (same clone as 13382-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 13382-1-AP (NCAPD2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13382-1-PBS in a different storage buffer formulation.

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  IP experiment of HEK-293 using 13382-1-AP (same clone as 13382-1-PBS)

  IP result of anti-NCAPD2 (IP:13382-1-AP, 4ug; Detection:13382-1-AP 1:600) with HEK-293 cells lysate 2800ug. This data was developed using the same antibody clone with 13382-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 13382-1-AP (same clone as 13382-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 13382-1-AP (NCAPD2 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13382-1-PBS in a different storage buffer formulation.

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  IHC staining of human skin cancer using 13382-1-AP (same clone as 13382-1-PBS)

  Immunohistochemical analysis of paraffin-embedded skin cancer slide using 13382-1-AP (NCAPD2 antibody) at dilution of 1:300 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13382-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 13382-1-AP (same clone as 13382-1-PBS)

  Immunofluorescent analysis of Hela cells, using NCAPD2 antibody 13382-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye). This data was developed using the same antibody clone with 13382-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 13382-1-AP (same clone as 13382-1-PBS)

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 13382-1-AP (NCAPD2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13382-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

13382-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA NCAPD2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NCAPD2 fusion protein Ag4219
• Vollständiger Name: non-SMC condensin I complex, subunit D2
• Berechnetes Molekulargewicht: 1401 aa, 157 kDa
• Beobachtetes Molekulargewicht: 157 kDa
• GenBank-Zugangsnummer: BC028182
• Gene symbol: NCAPD2
• Gene ID (NCBI): 9918
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NCAPD2 is a regulatory subunit of the condensin complex, which is required for conversion of interphase chromatin into mitotic-like condense chromosomes.  Condensin complexes are heteropentamers comprised of two SMC (structural maintenance of chromosomes) subunits and three non-SMC subunits. The C-terminus of NCAPD2 interacts with Histones H1 and H3 through their histone tails. A loss of NCAPD2 can lead to the disorganization of chromatid axes, misalignment of sister chromatids during metaphase and delayed entry into anaphase.