NCAPD3 Polyklonaler Antikörper

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NCAPD3 Polyklonal Antikörper für WB, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 16828-1-PBS

Synonyme

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  WB analysis of HepG2 using 16828-1-AP (same clone as 16828-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16828-1-AP (NCAPD3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16828-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 16828-1-AP (same clone as 16828-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 16828-1-AP (NCAPD3 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16828-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 16828-1-AP (same clone as 16828-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 16828-1-AP (NCAPD3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16828-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 16828-1-AP (same clone as 16828-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 16828-1-AP (NCAPD3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16828-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HeLa using 16828-1-AP (same clone as 16828-1-PBS)

  IP result of anti-NCAPD3 (IP:16828-1-AP, 4ug; Detection:16828-1-AP 1:500) with HeLa cells lysate 600ug. This data was developed using the same antibody clone with 16828-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 16828-1-AP (same clone as 16828-1-PBS)

  Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 16828-1-AP (NCAPD3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 16828-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

16828-1-PBS bindet in WB, IF/ICC, IP, Indirect ELISA NCAPD3 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NCAPD3 fusion protein Ag10429
• Vollständiger Name: non-SMC condensin II complex, subunit D3
• Berechnetes Molekulargewicht: 1498 aa, 169 kDa
• Beobachtetes Molekulargewicht: 165 kDa
• GenBank-Zugangsnummer: BC011408
• Gene symbol: NCAPD3
• Gene ID (NCBI): 23310
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Condensin complexes I and II play essential roles in mitotic chromosome assembly and segregation. Both condensins contain 2 invariant structural maintenance of chromosome (SMC) subunits, SMC2 and SMC4 , but they contain different sets of non-SMC subunits. NCAPD3 is 1 of 3 non-SMC subunits that define condensin II ([PMID: 14532007).