NCDN Rekombinanter Antikörper

NCDN Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

243146E2

Kat-Nr. : 85730-1-RR

Synonyme

Neurochondrin, KIAA0607, NEDIES

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  WB analysis using 85730-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85730-1-RR (NCDN antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 85730-1-RR

  Biolayer interferometry (BLl) kinetic assays of 85730-1-RR against Human NCDN were performed. The affinity constant is 1.35 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, fetales humanes Hirngewebe, Rattenhirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

85730-1-RR bindet in WB, ELISA NCDN und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: NCDN fusion protein Ag3859
• Vollständiger Name: neurochondrin
• Berechnetes Molekulargewicht: 729 aa, 79 kDa
• Beobachtetes Molekulargewicht: 75-79 kDa
• GenBank-Zugangsnummer: BC024592
• Gene symbol: Neurochondrin
• Gene ID (NCBI): 23154
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NCDN protein (Neurochondrin) is a highly conserved 79 kDa adaptor protein, which was first discovered in 1997 and mainly expressed in the nervous system. NCDN protein plays an important role in neuronal differentiation, formation and maintenance of dendritic spines. It is involved in the development of synapses, neuronal differentiation and signal transduction, and maintains cell polarity by regulating dendritic morphogenesis and neural signal pathway. Recent studies have found that NCDN protein is expressed not only in neurons, but also in myeloid cells (such as neutrophils), and plays a role as a negative regulator of innate immunity. Mice lacking NCDN in myeloid cells are more immune to bacterial infection because of the increased reactivity of neutrophils (PMID: 37503670). The molecular weight of NCDN is 78 kDa.