NCKAP1 Polyklonaler Antikörper

NCKAP1 Polyklonal Antikörper für WB, IHC, IF/ICC, IF-P, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 12140-1-PBS

Synonyme

NAP125, NAP1, NAP 1, Membrane-associated protein HEM-2, KIAA0587

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  WB analysis of mouse brain using 12140-1-AP (same clone as 12140-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12140-1-AP (NCKAP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12140-1-PBS in a different storage buffer formulation.

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  WB analysis using 12140-1-AP (same clone as 12140-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 12140-1-AP (NCKAP1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12140-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 12140-1-AP (same clone as 12140-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12140-1-AP (NAP1;NCKAP1 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 12140-1-PBS in a different storage buffer formulation.

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  IHC staining of human heart using 12140-1-AP (same clone as 12140-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 12140-1-AP (NAP1;NCKAP1 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 12140-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 12140-1-AP (same clone as 12140-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12140-1-AP (NAP1;NCKAP1 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12140-1-PBS in a different storage buffer formulation.

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  IHC staining of human heart using 12140-1-AP (same clone as 12140-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 12140-1-AP (NAP1;NCKAP1 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12140-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse brain using 12140-1-AP (same clone as 12140-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using NCKAP1 antibody (12140-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12140-1-PBS in a different storage buffer formulation.

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  IF Staining of U-251 using 12140-1-AP (same clone as 12140-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using NCKAP1 antibody (12140-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 12140-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

12140-1-PBS bindet in WB, IHC, IF/ICC, IF-P, Indirect ELISA NCKAP1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NCKAP1 fusion protein Ag2788
• Vollständiger Name: NCK-associated protein 1
• Berechnetes Molekulargewicht: 129 kDa
• Beobachtetes Molekulargewicht: 115-125 kDa
• GenBank-Zugangsnummer: BC015025
• Gene symbol: NCKAP1
• Gene ID (NCBI): 10787
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NCK-associated protein 1 (NCKAP1), also known as NAP1 or HEM2, is encoded by an apoptosis-related gene human NAP1, the expression of which is strongly down-regulated in sporadic Alzheimer's disease (AD). Human NAP1 is predominantly expressed in neuronal cells. Antisense oligo DNA of human NAP1 transcripts was found to induce apoptosis of neuronal cells. Loss of NAP1 function disrupts neuronal differentiation. Likewise, NAP1 plays an essential role in facilitating neuronal cytoskeletal changes underlying the postmigratory differentiation of cortical neurons, a critical step in functional wiring of the cortex. Human NAP1 is also proved to be an orthologue of rat Nap1 which binds to the adaptor molecule NCK in signal transduction.