NCKAP5 Polyklonaler Antikörper

NCKAP5 Polyklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 31199-1-AP

Synonyme

NAP5, Peripheral clock protein, Nck associated protein 5, NAP 5, ERIH2

---

• 

  WB analysis using 31199-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 31199-1-AP (NCKAP5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 31199-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 31199-1-AP (NCKAP5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IF Staining of U2OS using 31199-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using NCKAP5 antibody (31199-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, HepG2-Zellen
• Erfolgreiche Detektion in IF/ICC: U2OS-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

31199-1-AP bindet in WB, IF/ICC, ELISA NCKAP5 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NCKAP5 fusion protein Ag35092
• Vollständiger Name: Nck-associated protein 5
• Berechnetes Molekulargewicht: 209 kDa
• Beobachtetes Molekulargewicht: 250-260 kDa
• GenBank-Zugangsnummer: NM_207363
• Gene symbol: NAP5
• Gene ID (NCBI): 344148
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NCKAP5 (Nck-associated protein 5), also known as NAP5. NCKAP5 is predicted to be located in cells, especially in nucleoli, Golgi apparatus, cell membrane and cytoplasm. It is widely expressed in many tissues, especially in lung and kidney, and in more than 20 other tissues. And the expression of NCKAP5 was enhanced in oligodendrocytes, oligodendrocyte precursor cells and astrocytes. It may interact with other protein, such as NPM1 and centromere protein complex, and regulate accurate chromosome segregation and distribution. NCKAP5 may be involved in the formation and development of tumors, especially in the process of tumor variation such as ovary and breast cancer. In addition, NCKAP5 may be a diagnostic biomarker in non-small cell lung cancer (NSCLC), which is related to the infiltration of activated B cells, regulatory T cells (Tregs), neutrophils and dendritic cells (PMID: 36628881). The molecular weight of NCKAP5 is 208 kDa, it also has some protein modifications.