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NCKAP5 Polyklonaler Antikörper

NCKAP5 Polyklonal Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 31199-1-PBS

Synonyme

NAP5, Peripheral clock protein, Nck associated protein 5, NAP 5, ERIH2

Filter:
  • Western Blot (WB) analysis of various lysates using NCKAP5 Polyclonal antibody (31199-1-AP)

    WB analysis using 31199-1-AP (same clone as 31199-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 31199-1-AP (NCKAP5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31199-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using NCKAP5 Polyclonal antibody (31199-1-AP)

    WB analysis using 31199-1-AP (same clone as 31199-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 31199-1-AP (NCKAP5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31199-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using NCKAP5 Polyclonal antibody (31199-1-AP)

    IF Staining of U2OS using 31199-1-AP (same clone as 31199-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using NCKAP5 antibody (31199-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 31199-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

31199-1-PBS bindet in WB, IF/ICC, Indirect ELISA NCKAP5 und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen NCKAP5 fusion protein Ag35092
Vollständiger Name Nck-associated protein 5
Berechnetes Molekulargewicht209 kDa
Beobachtetes Molekulargewicht250-260 kDa
GenBank-ZugangsnummerNM_207363
Gene symbol NAP5
Gene ID (NCBI) 344148
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NCKAP5 (Nck-associated protein 5), also known as NAP5. NCKAP5 is predicted to be located in cells, especially in nucleoli, Golgi apparatus, cell membrane and cytoplasm. It is widely expressed in many tissues, especially in lung and kidney, and in more than 20 other tissues. And the expression of NCKAP5 was enhanced in oligodendrocytes, oligodendrocyte precursor cells and astrocytes. It may interact with other protein, such as NPM1 and centromere protein complex, and regulate accurate chromosome segregation and distribution. NCKAP5 may be involved in the formation and development of tumors, especially in the process of tumor variation such as ovary and breast cancer. In addition, NCKAP5 may be a diagnostic biomarker in non-small cell lung cancer (NSCLC), which is related to the infiltration of activated B cells, regulatory T cells (Tregs), neutrophils and dendritic cells (PMID: 36628881). The molecular weight of NCKAP5 is 208 kDa, it also has some protein modifications.