NCOA3 Polyklonaler Antikörper

NCOA3 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 29587-1-AP

Synonyme

AIB1, SRC3, ACTR, AIB 1, AIB-1

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  WB analysis using 29587-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29587-1-AP (NCOA3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 29587-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29587-1-AP (NCOA3 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 29587-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 29587-1-AP (NCOA3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human skin cancer using 29587-1-AP

  Immunohistochemical analysis of paraffin-embedded skin cancer slide using 29587-1-AP (NCOA3 antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 29587-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 29587-1-AP (NCOA3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 29587-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using NCOA3 antibody (29587-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Daudi-Zellen, HEK-293-Zellen, HeLa-Zellen, Jurkat-Zellen, K-562-Zellen, MCF-7-Zellen, PC-3-Zellen
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe, humanes Hautkrebsgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

29587-1-AP bindet in WB, IHC, IF/ICC, ELISA NCOA3 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NCOA3 fusion protein Ag30633
• Vollständiger Name: nuclear receptor coactivator 3
• Berechnetes Molekulargewicht: 1424 aa, 155 kDa
• Beobachtetes Molekulargewicht: 150-160 kDa
• GenBank-Zugangsnummer: BC119001
• Gene symbol: NCOA3
• Gene ID (NCBI): 8202
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NCOA3, also named as AIB1, SRC3 and TRAM1, belongs to the SRC/p160 nuclear receptor coactivator family. NCOA3 is a nuclear receptor coactivator that directly binds nuclear receptors and stimulates the transcriptional activities in a hormone-dependent fashion. NOCA3 plays a central role in creating a multisubunit coactivator complex, which probably acts via remodeling of chromatin. Involved in the coactivation of different nuclear receptors, such as for steroids (GR and ER), retinoids (RARs and RXRs), thyroid hormone (TRs), vitamin D3 (VDR) and prostanoids (PPARs). NCOA3 also involved in the coactivation of the NF-kappa-B pathway via its interaction with the NFKB1 subunit.