NCOR2 Polyklonaler Antikörper

NCOR2 Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 29952-1-PBS

Synonyme

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  WB analysis using 29952-1-AP (same clone as 29952-1-PBS)

  MOLT-4 cells were subjected to Tris-Acetate gel system followed by western blot with 29952-1-AP (NCOR2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29952-1-PBS in a different storage buffer formulation.

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  WB analysis using 29952-1-AP (same clone as 29952-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 29952-1-AP (NCOR2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29952-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 29952-1-AP (same clone as 29952-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 29952-1-AP (NCOR2 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29952-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 29952-1-AP (same clone as 29952-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 29952-1-AP (NCOR2 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29952-1-PBS in a different storage buffer formulation.

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  IF Staining of A549 using 29952-1-AP (same clone as 29952-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using NCOR2 antibody (29952-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 29952-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

29952-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA NCOR2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NCOR2 fusion protein Ag30727
• Vollständiger Name: nuclear receptor co-repressor 2
• Berechnetes Molekulargewicht: 274 kDa
• Beobachtetes Molekulargewicht: 274 kDa
• GenBank-Zugangsnummer: NM_006312
• Gene symbol: NCOR2
• Gene ID (NCBI): 9612
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NCOR2, nuclear receptor corepressor 2 , is a transcriptional corepressor that that contains several nuclear receptor-interacting domains. NCOR2/SMRT acts as a platform protein, facilitating the recruitment of histone deacetylases to the DNA promoters bound by its interacting transcription factors (PMID: 9150137). NCOR2 mediates the transcriptional repression activity of some nuclear receptors by promoting chromatin condensation, thus preventing access of the basal transcription (PMID: 23081895).