ND1 Polyklonaler Antikörper

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• KD/KO Validated

ND1 Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (3)

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

69

Rezensionen (3)

Kat-Nr. : 19703-1-AP

Synonyme

MT ND1, MTND1, NADH dehydrogenase subunit 1, NADH1, ND1

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Galerie der Validierungsdaten

• 

  WB analysis using 19703-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19703-1-AP (ND1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse liver using 19703-1-AP

  IP Result of anti-ND1 (IP:19703-1-AP, 3ug; Detection:19703-1-AP 1:500) with mouse liver tissue lysate 4000ug.

• 

  IHC staining of human liver using 19703-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 19703-1-AP (ND1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 19703-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 19703-1-AP (ND1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslebergewebe, mouse liver mitochondria
• Erfolgreiche IP: Mauslebergewebe
• Erfolgreiche Detektion in IHC: humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 66 publications below
• IHC: See 4 publications below
• IF: See 1 publications below

Produktinformation

19703-1-AP bindet in WB, IP, IHC, IF, ELISA ND1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, hamster, Hausschwein, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: NADH dehydrogenase, subunit 1 (complex I)
• Berechnetes Molekulargewicht: 36 kDa
• Beobachtetes Molekulargewicht: 28-38 kDa
• GenBank-Zugangsnummer: NC_012920
• Gene symbol: ND1
• Gene ID (NCBI): 4535
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ND1 belongs to the complex I subunit 1 family. ND1 is a core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.

Publikationen

Species	Application	Title
human	IF	Cell; Single-Cell Transcriptomic Atlas of Primate Ovarian Aging.; Authors - Si Wang
mouse	WB	Cell; MicroRNA Directly Enhances Mitochondrial Translation during Muscle Differentiation.; Authors - Xiaorong Zhang ; KD Validated
mouse	IHC	Circulation; Extracellular Tuning of Mitochondrial Respiration Leads to Aortic Aneurysm.; Authors - Jorge Oller
human	WB	Mol Cell; Filamentous GLS1 promotes ROS-induced apoptosis upon glutamine deprivation via insufficient asparagine synthesis.; Authors - Bin Jiang
human	WB	Sci Adv; MCART1/SLC25A51 is required for mitochondrial NAD transport.; Authors - Nora Kory
mouse	WB	Nat Commun; Disuse-associated loss of the protease LONP1 in muscle impairs mitochondrial function and causes reduced skeletal muscle mass and strength.; Authors - Zhisheng Xu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Tania (Verified Customer) (03-05-2022)

a lot of background. difficult to say each band is the correct one.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: ES2 human cells

FH

Daniel (Verified Customer) (10-01-2019)

ND2 was detected in both liver (L) and heart (H) lysate from a wildtype mouse. No ND1 was detected in samples processed in parallel.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mouse liver (L), heart (H)

ND1 Antibody Western Blot, validation (1:1000 dilution) in Mouse liver (L), heart (H) (Cat no:19703-1-AP)

FH

Loukmane (Verified Customer) (08-10-2018)

It works very well with A549 and lung tissue, although with the former the background is higherThe image shows ND1 in whole A549 lysate

• Applications: Western Blot,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Lung tissue and A549

ND1 Antibody Western Blot, validation (1:500 dilution) in Lung tissue and A549 (Cat no:19703-1-AP)

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