ND4 Rekombinanter Antikörper

ND4 Rekombinant Antikörper für WB, IHC, Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

242422E2

Kat-Nr. : 85098-1-PBS

Synonyme

MT-ND4, 242422E2, MT ND4, MTND4, NADH4

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  WB analysis using 85098-1-RR (same clone as 85098-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85098-1-RR (ND4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85098-1-PBS in a different storage buffer formulation.

• 

  Sandwich ELISA standard curve of MP02094-1

  Sandwich ELISA standard curve of MP02094-1, Human ND4 Recombinant Matched Antibody Pair - PBS only. 85098-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard SY01674. 85098-2-PBS was HRP conjugated as the detection antibody. Range: 12.5-800 pg/mL.

• 

  Sample test of MP02094-1

  The mean ND4 concentration was determined to be 23.9 ng/mL in mouse liver tissue extract based on a 3.7 mg/mL extract load.

• 

  IHC staining of human stomach cancer using 85098-1-RR (same clone as 85098-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 85098-1-RR (ND4 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85098-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach cancer using 85098-1-RR (same clone as 85098-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 85098-1-RR (ND4 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85098-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

85098-1-PBS bindet in WB, IHC, Sandwich ELISA, Indirect ELISA, Sample test ND4 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: NADH dehydrogenase, subunit 4 (complex I)
• Berechnetes Molekulargewicht: 52 kDa
• Beobachtetes Molekulargewicht: 43-52 kDa
• GenBank-Zugangsnummer: YP_003024035
• Gene symbol: ND4
• Gene ID (NCBI): 4538
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.