MT-ND5 Polyklonaler Antikörper

MT-ND5 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (2)

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(36)

Rezensionen (1)

Kat-Nr. : 55410-1-AP

Synonyme

ND5, ND 5, NADH5, MTND5, MT ND5

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  WB analysis using 55410-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 55410-1-AP (ND5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 55410-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55410-1-AP (MT-ND5 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver using 55410-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 55410-1-AP (ND5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 55410-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 55410-1-AP (ND5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslebergewebe, Rattenlebergewebe
• Erfolgreiche Detektion in IHC: humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 36 publications below

Produktinformation

55410-1-AP bindet in WB, IHC, ELISA MT-ND5 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, hamster, Hausschwein, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: NADH dehydrogenase, subunit 5 (complex I)
• Berechnetes Molekulargewicht: 67 kDa
• Beobachtetes Molekulargewicht: 65-70 kDa
• GenBank-Zugangsnummer: AF465942
• Gene symbol: MT-ND5
• Gene ID (NCBI): 4540
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ND5 belongs to the complex I subunit 1 family. ND5 is a core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.

Publikationen

Species	Application	Title
human	WB	Cell Metab; Malic enzyme 2 connects the Krebs cycle intermediate fumarate to mitochondrial biogenesis.; Authors - Yi-Ping Wang
human	WB	Mol Cell; Hepatic micropeptide modulates mitochondrial RNA processing machinery in hepatocellular carcinoma; Authors - Linyu Zhu
human	WB	Mol Cell; Filamentous GLS1 promotes ROS-induced apoptosis upon glutamine deprivation via insufficient asparagine synthesis.; Authors - Bin Jiang
human	WB	Sci Adv; MCART1/SLC25A51 is required for mitochondrial NAD transport.; Authors - Nora Kory
human	WB	EMBO J; EglN2 associates with the NRF1-PGC1α complex and controls mitochondrial function in breast cancer.; Authors - Jing Zhang
human	WB	EMBO Rep; MITRAC15/COA1 promotes mitochondrial translation in a ND2 ribosome-nascent chain complex.; Authors - Cong Wang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Baptiste (Verified Customer) (05-23-2025)

Working well but have a light unspecific (?) band close to ND5.

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Lymphoblastoid

MT-ND5 Antibody Western Blot validation (1:500 dilution) in Lymphoblastoid (Cat no:55410-1-AP)