NDC80 Polyklonaler Antikörper

NDC80 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(6)

Kat-Nr. : 18932-1-AP

Synonyme

Kinetochore-associated protein 2, Kinetochore protein Hec1, hsNDC80, HsHec1, HEC1

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  WB analysis using 18932-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18932-1-AP (NDC80 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human spleen using 18932-1-AP

  human spleen tissue were subjected to SDS PAGE followed by western blot with 18932-1-AP (NDC80 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of A2780 using 18932-1-AP

  A2780 cells were subjected to SDS PAGE followed by western blot with 18932-1-AP (NDC80 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of A2780 using 18932-1-AP

  A2780 cells were subjected to SDS PAGE followed by western blot with 18932-1-AP (NDC80 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of A2780 using 18932-1-AP

  IP result of anti-NDC80 (IP:18932-1-AP, 4ug; Detection:18932-1-AP 1:500) with A2780 cells lysate 960ug.

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  IHC staining of human brain using 18932-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 18932-1-AP (NDC80 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human brain using 18932-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 18932-1-AP (NDC80 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of A549 using 18932-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using NDC80 antibody (18932-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, A2780-Zellen, humanes Milzgewebe, Jurkat-Zellen, Maushodengewebe
• Erfolgreiche IP: A2780-Zellen
• Erfolgreiche Detektion in IHC: humanes Hirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: A549-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 4 publications below
• IHC: See 2 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Produktinformation

18932-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA NDC80 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NDC80 fusion protein Ag8065
• Vollständiger Name: NDC80 homolog, kinetochore complex component (S. cerevisiae)
• Berechnetes Molekulargewicht: 74 kDa
• Beobachtetes Molekulargewicht: 76 kDa
• GenBank-Zugangsnummer: BC035617
• Gene symbol: NDC80
• Gene ID (NCBI): 10403
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	IP	J Cell Biol; PP6 regulation of Aurora A-TPX2 limits NDC80 phosphorylation and mitotic spindle size; Authors - Tomoaki Sobajima
human	WB	Oncotarget; Co-expression of mitosis-regulating genes contributes to malignant progression and prognosis in oligodendrogliomas.; Authors - Yanwei Liu
human	WB,IHC	Front Pharmacol; Upregulation of nuclear division cycle 80 contributes to therapeutic resistance via the promotion of autophagy-related protein-7-dependent autophagy in lung cancer; Authors - Xi Chen ; KD Validated
human	WB	Onco Targets Ther; Targeting DTL induces cell cycle arrest and senescence and suppresses cell growth and colony formation through TPX2 inhibition in human hepatocellular carcinoma cells.; Authors - Yu-Chia Chen
	IHC	iScience; NDC80 status pinpoints mitotic kinase inhibitors as emerging therapeutic options in clear cell renal cell carcinoma; Authors - Cheng Hu
human	WB,IF	CNS Neurosci Ther; NDC80/HEC1 promotes macrophage polarization and predicts glioma prognosis via single-cell RNA-seq and in vitro experiment; Authors - Weijie Ye ; KD Validated