NDE1 Rekombinanter Antikörper

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• KD/KO Validated

NDE1 Rekombinant Antikörper für WB, IHC, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

242340H4

Kat-Nr. : 84799-4-PBS

Synonyme

242340H4, HOM TES 87, NudE, NUDE1

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  WB analysis using 84799-4-RR (same clone as 84799-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84799-4-RR (NDE1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84799-4-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 84799-4-RR (same clone as 84799-4-PBS)

  WB result of NDE1 antibody (84799-4-RR; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NDE1 transfected HepG2 cells. This data was developed using the same antibody clone with 84799-4-PBS in a different storage buffer formulation.

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  Sandwich ELISA sample test of MP01589-1

  The mean NDE1 concentration was determined to be 38.38 ng/mL in HepG2 cell extract based on a 2.50 mg/mL extract load, 43.20 ng/mL in K-562 cell extract based on a 1.00 mg/mL extract load and 22.42 ng/mL in HeLa cell extract based on a 1.50 mg/mL extract load.

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  Sandwich ELISA standard curve of MP01589-1

  Sandwich ELISA standard curve of MP01589-1, Human NDE1 Recombinant Matched Antibody Pair - PBS only. 84799-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag0336. 84799-4-PBS was HRP conjugated as the detection antibody. Range: 0.156-10 ng/mL.

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  IHC staining of mouse liver using 84799-4-RR (same clone as 84799-4-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84799-4-RR (NDE1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84799-4-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 84799-4-RR (same clone as 84799-4-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84799-4-RR (NDE1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84799-4-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 84799-4-RR (same clone as 84799-4-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using NDE1 antibody (84799-4-RR, Clone: 242340H4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84799-4-PBS in a different storage buffer formulation.

• 

  Cytometric bead array standard curve of MP01589-1

  Cytometric bead array standard curve of MP01589-1, NDE1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84799-2-PBS. Detection antibody: 84799-4-PBS. Standard: Ag0336. Range: 0.313-40 ng/mL.

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  Affinity and Kinetic Characterization of 84799-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84799-4-RR against Human NDE1 were performed. The affinity constant is 0.342 nM.

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Geprüfte Anwendungen

Produktinformation

84799-4-PBS bindet in WB, IHC, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA NDE1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: NDE1 fusion protein Ag0336
• Vollständiger Name: nudE nuclear distribution gene E homolog 1 (A. nidulans)
• Berechnetes Molekulargewicht: 335 aa, 38 kDa
• Beobachtetes Molekulargewicht: 40 kDa
• GenBank-Zugangsnummer: BC001421
• Gene symbol: NDE1
• Gene ID (NCBI): 54820
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NDE1 (NUDE /NUDE1) is a LIS1-interacting protein. A signaling pathway containing NDE1, LIS1, and cytoplasmic dynein is conserved for eukaryotic nuclear migration and has profound impact on neuronal migration during development of the central nervous system. LIS1 and dynein have been shown to be involved in mitosis and NDE1 participates in a subset of dynein function in M phase. NDE1 is specifically phosphorylated in M phase in its serine/threonine phosphorylation motifs, probably by Cdc2 and also Erk1 and Erk2. A fraction of NDEl binds to centrosomes strongly in interphase and is localized to mitotic spindles in early M phase. Phosphorylation of NDE1 also regulates the cell-cycle-dependent distribution, possibly by increasing its dissociation rate at the microtubule-organizing center.