NDE1 Rekombinanter Antikörper

• Featured Product
• KD/KO Validated

NDE1 Rekombinant Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

242340H4

Kat-Nr. : 84799-4-RR

Synonyme

242340H4, HOM TES 87, NudE, NUDE1

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  WB analysis using 84799-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84799-4-RR (NDE1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 84799-4-RR

  WB result of NDE1 antibody (84799-4-RR; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NDE1 transfected HepG2 cells.

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  IHC staining of mouse liver using 84799-4-RR

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84799-4-RR (NDE1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 84799-4-RR

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84799-4-RR (NDE1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 84799-4-RR

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using NDE1 antibody (84799-4-RR, Clone: 242340H4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 84799-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84799-4-RR against Human NDE1 were performed. The affinity constant is 0.342 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, HEK-293-Zellen, humanes Herzgewebe, Maushirngewebe, Rattenhirngewebe, SKOV-3-Zellen
• Erfolgreiche Detektion in IHC: Mauslebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84799-4-RR bindet in WB, IHC, IF/ICC, ELISA NDE1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: NDE1 fusion protein Ag0336
• Vollständiger Name: nudE nuclear distribution gene E homolog 1 (A. nidulans)
• Berechnetes Molekulargewicht: 335 aa, 38 kDa
• Beobachtetes Molekulargewicht: 40 kDa
• GenBank-Zugangsnummer: BC001421
• Gene symbol: NDE1
• Gene ID (NCBI): 54820
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NDE1 (NUDE /NUDE1) is a LIS1-interacting protein. A signaling pathway containing NDE1, LIS1, and cytoplasmic dynein is conserved for eukaryotic nuclear migration and has profound impact on neuronal migration during development of the central nervous system. LIS1 and dynein have been shown to be involved in mitosis and NDE1 participates in a subset of dynein function in M phase. NDE1 is specifically phosphorylated in M phase in its serine/threonine phosphorylation motifs, probably by Cdc2 and also Erk1 and Erk2. A fraction of NDEl binds to centrosomes strongly in interphase and is localized to mitotic spindles in early M phase. Phosphorylation of NDE1 also regulates the cell-cycle-dependent distribution, possibly by increasing its dissociation rate at the microtubule-organizing center.