NDFIP1 Polyklonaler Antikörper

NDFIP1 Polyklonal Antikörper für WB, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

Kat-Nr. : 15602-1-AP

Synonyme

PSEC0223, PSEC0192, NEDD4 WW domain-binding protein 5, NEDD4 family-interacting protein 1, N4WBP5

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  WB analysis using 15602-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15602-1-AP (NDFIP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 15602-1-AP

  IP result of anti-NDFIP1 (IP:15602-1-AP, 3ug; Detection:15602-1-AP 1:300) with HEK-293 cells lysate 3200ug.

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  IF Staining of HEK-293 using 15602-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using NDFIP1 antibody (15602-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, SH-SY5Y-Zellen
• Erfolgreiche IP: HEK-293-Zellen
• Erfolgreiche Detektion in IF/ICC: HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• IP: See 2 publications below

Produktinformation

15602-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA NDFIP1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NDFIP1 fusion protein Ag7985
• Vollständiger Name: Nedd4 family interacting protein 1
• Berechnetes Molekulargewicht: 25 kDa
• Beobachtetes Molekulargewicht: 25-30 kDa
• GenBank-Zugangsnummer: BC004317
• Gene symbol: NDFIP1
• Gene ID (NCBI): 80762
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Ndfip1 is an adaptor protein for the Nedd4 family of ubiquitin ligases and has been found to be upregulated in neurons after brain injury, including head trauma, stroke and metal toxicity. Recent studies confirmed the role of Ndfip1 as a regulator of iron metabolism and pointed out that Ndfip1 was involved in iron homeostasis by regulating the degradation of iron importer divalent metal transporter 1. Ndfip1 is a transmembrane protein that is localized to the Golgi and post-Golgi vesicles such as endosomes. It is an adaptor protein that recruits E3 ligases to ubiquitinate target proteins. It is ubiquitously expressed and contains 2 PY motifs, which interact with several Nedd4 family E3s to degradate proteins.

Publikationen

Species	Application	Title
human	IP	Mol Cell; PKCλ/ι inhibition activates an ULK2-mediated interferon response to repress tumorigenesis.; Authors - Juan F Linares
human,rat	WB,IHC,IF	J Neurooncol; Cabergoline-induced NDFIP1 upregulation in pituitary neuroendocrine tumor cells activates mTOR signaling and contributes to cabergoline resistance; Authors - Weiting Gu
human	WB,IF,IP	Cell Death Discov; WWP1 upregulation predicts poor prognosis and promotes tumor progression by regulating ubiquitination of NDFIP1 in intrahepatic cholangiocarcinoma.; Authors - Yongjian Li
human	WB	Nat Chem Biol; µMap proximity labeling in living cells reveals stress granule disassembly mechanisms; Authors - Chenmengxiao Roderick Pan ; KD Validated