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NDFIP1 Polyklonaler Antikörper

NDFIP1 Polyklonal Antikörper für WB, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 15602-1-PBS

Synonyme

Filter:
  • Western Blot (WB) analysis of various lysates using NDFIP1 Polyclonal antibody (15602-1-AP)

    WB analysis using 15602-1-AP (same clone as 15602-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 15602-1-AP (NDFIP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15602-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using NDFIP1 Polyclonal antibody (15602-1-AP)

    IP experiment of HEK-293 using 15602-1-AP (same clone as 15602-1-PBS)

    IP result of anti-NDFIP1 (IP:15602-1-AP, 3ug; Detection:15602-1-AP 1:300) with HEK-293 cells lysate 3200ug. This data was developed using the same antibody clone with 15602-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using NDFIP1 Polyclonal antibody (15602-1-AP)

    IF Staining of HEK-293 using 15602-1-AP (same clone as 15602-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using NDFIP1 antibody (15602-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 15602-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

15602-1-PBS bindet in WB, IF/ICC, IP, Indirect ELISA NDFIP1 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen NDFIP1 fusion protein Ag7985
Vollständiger Name Nedd4 family interacting protein 1
Berechnetes Molekulargewicht 25 kDa
Beobachtetes Molekulargewicht 25-30 kDa
GenBank-ZugangsnummerBC004317
Gene symbol NDFIP1
Gene ID (NCBI) 80762
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Ndfip1 is an adaptor protein for the Nedd4 family of ubiquitin ligases and has been found to be upregulated in neurons after brain injury, including head trauma, stroke and metal toxicity. Recent studies confirmed the role of Ndfip1 as a regulator of iron metabolism and pointed out that Ndfip1 was involved in iron homeostasis by regulating the degradation of iron importer divalent metal transporter 1. Ndfip1 is a transmembrane protein that is localized to the Golgi and post-Golgi vesicles such as endosomes. It is an adaptor protein that recruits E3 ligases to ubiquitinate target proteins. It is ubiquitously expressed and contains 2 PY motifs, which interact with several Nedd4 family E3s to degradate proteins.