NDNL2 Polyklonaler Antikörper

NDNL2 Polyklonal Antikörper für IF, IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

1

Kat-Nr. : 27488-1-AP

Synonyme

HCA4, MAGE G1 antigen, MAGEG1, MAGEL3, Melanoma associated antigen G1, NDNL2, necdin like 2, Necdin like protein 2, NSE3, NSMCE3

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Galerie der Validierungsdaten

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  WB analysis of HepG2 using 27488-1-AP

  HepG2, L02 and HeLa cells were subjected to SDS PAGE followed by western blot with 27488-1-AP (NDNL2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 27488-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 27488-1-AP (NDNL2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 27488-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 27488-1-AP (NDNL2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 27488-1-AP

  HepG2 cells and human placenta tissue were subjected to SDS PAGE followed by western blot with 27488-1-AP (NDNL2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 27488-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 27488-1-AP (NDNL2 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 27488-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 27488-1-AP (NDNL2 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 27488-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 27488-1-AP (NDNL2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, HeLa-Zellen, humanes Plazenta-Gewebe, L02-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

27488-1-AP bindet in WB, IHC, IF, ELISA NDNL2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NDNL2 fusion protein Ag26663
• Vollständiger Name: necdin-like 2
• Berechnetes Molekulargewicht: 34 kDa
• Beobachtetes Molekulargewicht: 38 kDa
• GenBank-Zugangsnummer: BC053999
• Gene symbol: NDNL2
• Gene ID (NCBI): 56160
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NDNL2 (also known as NSMCE3 or MAGEG1) is a part of the SMC5-6 protein complex that is essential for DNA damage response and chromosome segregation (PMID: 18086888; 27427983). The gene of human NDNL2 maps to chromosome 15q13.1. Northern blot analysis detected expression of a 1.9-kb transcript in all human tissues tested, with highest expression in testis (PMID: 11782285). Missense mutations in NSMCE3 have been associated with an autosomal recessive chromosome breakage syndrome that leads to defective T and B cell function and acute respiratory distress syndrome in early childhood (PMID: 27427983).

Publikationen

Species	Application	Title
human	WB	Cell Rep; Epstein-Barr virus BNRF1 destabilizes SMC5/6 cohesin complexes to evade its restriction of replication compartments.; Authors - Stephanie Pei Tung Yiu