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NDP52 Rekombinanter Antikörper

NDP52 Rekombinant Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

250714E9

Kat-Nr. : 86167-1-PBS

Synonyme

CALCOCO2, Calcium-binding and coiled-coil domain-containing protein 2, Nuclear domain 10 protein 52, Nuclear dot protein 52

Filter:
  • Western Blot (WB) analysis of various lysates using NDP52 Recombinant antibody (86167-1-RR)

    WB analysis using 86167-1-RR (same clone as 86167-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86167-1-RR (NDP52 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86167-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using NDP52 Recombinant antibody (86167-1-RR)

    IF Staining of HeLa using 86167-1-RR (same clone as 86167-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using NDP52 antibody (86167-1-RR, Clone: 250714E9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86167-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 86167-1-RR

    Affinity and Kinetic Characterization of 86167-1-RR

    Biolayer interferometry (BLl) kinetic assays of 86167-1-RR against Human NDP52 were performed. The affinity constant is 0.643 nM.

Geprüfte Anwendungen

Produktinformation

86167-1-PBS bindet in WB, IF/ICC, Indirect ELISA NDP52 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen NDP52 fusion protein Ag2866
Vollständiger Name calcium binding and coiled-coil domain 2
Berechnetes Molekulargewicht 446 aa, 52 kDa
Beobachtetes Molekulargewicht52 kDa
GenBank-ZugangsnummerBC015893
Gene symbol NDP52
Gene ID (NCBI) 10241
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NDP52, also named as CALCOCO2, is an autophagy receptor. It plays a role in ruffle formation and actin cytoskeleton organization. Mouse/Rat NDP52 has some isoforms with MW 28-40 kDa and 67 kDa. Human NDP52 has some isoforms with MW 43-47 kDa and 52-55 kDa.