NDP52 Rekombinanter Antikörper

NDP52 Rekombinant Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

250714E9

Kat-Nr. : 86167-1-RR

Synonyme

CALCOCO2, Calcium-binding and coiled-coil domain-containing protein 2, Nuclear domain 10 protein 52, Nuclear dot protein 52

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  WB analysis using 86167-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86167-1-RR (NDP52 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 86167-1-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using NDP52 antibody (86167-1-RR, Clone: 250714E9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 86167-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86167-1-RR against Human NDP52 were performed. The affinity constant is 0.643 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, A549-Zellen, Jurkat-Zellen
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

86167-1-RR bindet in WB, IF/ICC, ELISA NDP52 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: NDP52 fusion protein Ag2866
• Vollständiger Name: calcium binding and coiled-coil domain 2
• Berechnetes Molekulargewicht: 446 aa, 52 kDa
• Beobachtetes Molekulargewicht: 52 kDa
• GenBank-Zugangsnummer: BC015893
• Gene symbol: NDP52
• Gene ID (NCBI): 10241
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NDP52, also named as CALCOCO2, is an autophagy receptor. It plays a role in ruffle formation and actin cytoskeleton organization. Mouse/Rat NDP52 has some isoforms with MW 28-40 kDa and 67 kDa. Human NDP52 has some isoforms with MW 43-47 kDa and 52-55 kDa.