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NDRG3 Polyklonaler Antikörper

NDRG3 Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 31421-1-PBS

Synonyme

Protein NDRG3

Filter:
  • Western Blot (WB) analysis of various lysates using NDRG3 Polyclonal antibody (31421-1-AP)

    WB analysis using 31421-1-AP (same clone as 31421-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 31421-1-AP (NDRG3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31421-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A549 cells using NDRG3 Polyclonal antibody (31421-1-AP)

    WB analysis of A549 using 31421-1-AP (same clone as 31421-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 31421-1-AP (NDRG3 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31421-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using NDRG3 Polyclonal antibody (31421-1-AP)

    IHC staining of human colon using 31421-1-AP (same clone as 31421-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 31421-1-AP (NDRG3 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31421-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

31421-1-PBS bindet in WB, IHC, Indirect ELISA NDRG3 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen NDRG3 fusion protein Ag33626
Vollständiger Name NDRG family member 3
Berechnetes Molekulargewicht41 kDa
Beobachtetes Molekulargewicht41-45 kDa
GenBank-ZugangsnummerNM_032013
Gene symbol NDRG3
Gene ID (NCBI) 57446
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NDRG (N-Myc downstream-regulated gene) family is generally divided into two subfamilies, one composed of NDRG1 and NDRG3 with a homology of 67%, and the other composed of NDRG2 and NDRG4 with a homology of 58%. NDRG3 is a downstream regulatory factor of MYC, with a total length of 2588 base pairs. It is mainly expressed in ovary, prostate, testis, brain, spinal cord, thymus, heart and kidney. NDRG3 is located on chromosome 20q11.21-11.23 and encodes at least two subtypes, 375 and 363 amino acids, respectively, with an apparent molecular weight of 41 and 40 kDa, respectively. (PMID: 36619553)