NDUFA1 Polyklonaler Antikörper

NDUFA1 Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 15561-1-PBS

Synonyme

CI MWFE, CI-MWFE, Complex I MWFE, Complex I-MWFE, MWFE

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  WB analysis using 15561-1-AP (same clone as 15561-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 15561-1-AP (NDUFA1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15561-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 15561-1-AP (same clone as 15561-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 15561-1-AP (NDUFA1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15561-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse liver using 15561-1-AP (same clone as 15561-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 15561-1-AP (NDUFA1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15561-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse liver using 15561-1-AP (same clone as 15561-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 15561-1-AP (NDUFA1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15561-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse heart using 15561-1-AP (same clone as 15561-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 15561-1-AP (NDUFA1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15561-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse heart using 15561-1-AP (same clone as 15561-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 15561-1-AP (NDUFA1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15561-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

15561-1-PBS bindet in WB, IHC, Indirect ELISA NDUFA1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NDUFA1 fusion protein Ag7206
• Vollständiger Name: NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 1, 7.5kDa
• Berechnetes Molekulargewicht: 7.5 kDa
• Beobachtetes Molekulargewicht: 8 kDa
• GenBank-Zugangsnummer: BC000266
• Gene symbol: NDUFA1
• Gene ID (NCBI): 4694
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The NDUFA1 gene encoding the MWFE polypeptide is located on the X chromosome. The NDUFA1 gene product (MWFE protein) is essential for activity of complex I in mammalian mitochondria, and it is predicted to be a membrane protein (PMID: 10200266).