NDUFA1 Rekombinanter Antikörper

NDUFA1 Rekombinant Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

251084A5

Kat-Nr. : 86470-1-RR

Synonyme

CI MWFE, CI-MWFE, Complex I MWFE, Complex I-MWFE, MWFE

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  WB analysis using 86470-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86470-1-RR (NDUFA1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse heart using 86470-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 86470-1-RR (NDUFA1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse heart using 86470-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 86470-1-RR (NDUFA1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat heart using 86470-1-RR

  Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 86470-1-RR (NDUFA1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat heart using 86470-1-RR

  Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 86470-1-RR (NDUFA1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 86470-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86470-1-RR against Human NDUFA1 were performed. The affinity constant is 0.588 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A-673 cells, HeLa-Zellen, Mausherzgewebe, Ramos-Zellen, Rattenherzgewebe
• Erfolgreiche Detektion in IHC: Mausherzgewebe, Rattenherzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

86470-1-RR bindet in WB, IHC, ELISA NDUFA1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: NDUFA1 fusion protein Ag7206
• Vollständiger Name: NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 1, 7.5kDa
• Berechnetes Molekulargewicht: 7.5 kDa
• Beobachtetes Molekulargewicht: 8 kDa
• GenBank-Zugangsnummer: BC000266
• Gene symbol: NDUFA1
• Gene ID (NCBI): 4694
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NDUFA1, also known as NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1, it's part of the NADH dehydrogenase (ubiquinone) 1 alpha subcomplex. The NDUFA1 gene encoding the MWFE polypeptide is located on the X chromosome. The NDUFA1 gene product (MWFE protein) is essential for activity of complex I in mammalian mitochondria, and it is predicted to be a membrane protein (PMID: 10200266).