GRIM19 Polyklonaler Antikörper

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• KD/KO Validated

GRIM19 Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

8

Kat-Nr. : 10986-1-AP

Synonyme

B16.6, CDA016, CGI 39, CI B16.6, Complex I B16.6, GRIM 19, GRIM19, NDUFA13

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Galerie der Validierungsdaten

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  WB analysis using 10986-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10986-1-AP (GRIM19 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 10986-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 10986-1-AP (GRIM19 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 10986-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10986-1-AP (GRIM19 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 10986-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 10986-1-AP (GRIM19 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 10986-1-AP

  IP Result of anti-GRIM19 (IP:10986-1-AP, 4ug; Detection:10986-1-AP 1:500) with HEK-293 cells lysate 2000ug.

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  IHC staining of human prostate cancer using 10986-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10986-1-AP (GRIM19 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human prostate cancer using 10986-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10986-1-AP (GRIM19 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human kidney using 10986-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 10986-1-AP (GRIM19 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 10986-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 10986-1-AP (GRIM19 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human heart using 10986-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 10986-1-AP (GRIM19 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 10986-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 10986-1-AP (GRIM19 antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausherzgewebe, HEK-293-Zellen, MCF-7-Zellen, Rattenherzgewebe
• Erfolgreiche IP: HEK-293-Zellen
• Erfolgreiche Detektion in IHC: humanes Prostatakarzinomgewebe, humanes Herzgewebe, humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 7 publications below
• IHC: See 4 publications below

Produktinformation

10986-1-AP bindet in WB, IP, IHC, ELISA GRIM19 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GRIM19 fusion protein Ag1435
• Vollständiger Name: NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 13
• Berechnetes Molekulargewicht: 16.5 kDa
• Beobachtetes Molekulargewicht: 16-18 kDa
• GenBank-Zugangsnummer: BC009189
• Gene symbol: NDUFA13
• Gene ID (NCBI): 51079
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GRIM19(Gene associated with retinoic and interferon-induced mortality 19 protein) is also named as NDUFA13 ,CI-B16.6 and belongs to the complex I NDUFA13 subunit family.t GRIM-19 may distribute in the mitochondria and/or nucleus depending on the cell type.Alternatively, its cellular localization may be regulated by post-translational modifications such as phosphorylation and acetylation. Lastly, GRIM-19 may exist in different isoforms that may be distributed in distinct cellular locations(PMID:12628925).

Publikationen

Species	Application	Title
human	WB	Dev Cell; PDGF signaling inhibits mitophagy in glioblastoma stem cells through N6-methyladenosine.; Authors - Deguan Lv
human	WB,IHC	Hum Gene Ther; Nonviral delivery of GRIM-19 gene inhibits tumor growth with reduced local and systemic complications.; Authors - Yang Xu
human	WB,IHC	Exp Cell Res; GRIM-19 inhibits proliferation and induces apoptosis in a p53-dependent manner in colorectal cancer cells through the SIRT7/PCAF/MDM2 axis.; Authors - Ding Wang ; KD Validated
human	WB,IHC	Cancer Chemother Pharmacol; PMID: 30032449; Authors - Feng Ni
		Int J Oncol; Atrazine promotes RM1 prostate cancer cell proliferation by activating STAT3 signaling.; Authors - Kebang Hu
human	WB,IHC	Oncol Lett; GRIM-19 deficiency promotes clear cell renal cell carcinoma progression and is associated with high TNM stage and Fuhrman grade.; Authors - Naimeng Yan