NDUFA3 Polyklonaler Antikörper

NDUFA3 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(5)

Kat-Nr. : 17257-1-AP

Synonyme

NADH-ubiquinone oxidoreductase B9 subunit, Complex I-B9, CI-B9, CIB9, CI B9

---

• 

  WB analysis of human brain using 17257-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 17257-1-AP (NDUFA3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 17257-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17257-1-AP (NDUFA3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver using 17257-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17257-1-AP (NDUFA3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Sodium Citrate buffer (pH 6.0).

• 

  IHC staining of human stomach using 17257-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 17257-1-AP (NDUFA3 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 17257-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17257-1-AP (NDUFA3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Sodium Citrate buffer (pH 6.0).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Hirngewebe, HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 5 publications below

Produktinformation

17257-1-AP bindet in WB, IHC, ELISA NDUFA3 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NDUFA3 fusion protein Ag10912
• Vollständiger Name: NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 3, 9kDa
• Berechnetes Molekulargewicht: 65aa,7 kDa; 84aa,9 kDa
• Beobachtetes Molekulargewicht: 9 kDa
• GenBank-Zugangsnummer: BC011021
• Gene symbol: NDUFA3
• Gene ID (NCBI): 4696
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	iScience; Mutational burden of XPNPEP3 leads to defects in mitochondrial complex I and cilia in NPHPL1; Authors - Lingxiao Tong
human	WB	J Biol Chem; Complex I mutations synergize to worsen the phenotypic expression of Leber's hereditary optic neuropathy.; Authors - Yanchun Ji
human	WB	Invest Ophthalmol Vis Sci; Assocation Between Leber's Hereditary Optic Neuropathy and MT-ND1 3460G>A Mutation-Induced Alterations in Mitochondrial Function, Apoptosis, and Mitophagy.; Authors - Juanjuan Zhang
human	WB	Sci Rep; HDAC/H3K27ac-mediated transcription of NDUFA3 exerts protective effects on high glucose-treated human nucleus pulposus cells through improving mitochondrial function; Authors - Cheng Zheng
human	WB	Cell Stem Cell; Selective translation of nuclear mitochondrial respiratory proteins reprograms succinate metabolism in AML development and chemoresistance; Authors - Guoqiang Han