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  • Featured Product
  • KD/KO Validated

NDUFA4L2 Polyklonaler Antikörper

NDUFA4L2 Polyklonal Antikörper für FC (Intra)

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

FC (Intra)

Konjugation

CoraLite® Plus 488 Fluorescent Dye

Kat-Nr. : CL488-16480

Synonyme

FLJ26118, NDUFA4L2, NUOMS

Galerie der Validierungsdaten

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  • Flow cytometry (FC) experiment of HepG2 cells using CoraLite® Plus 488-conjugated NDUFA4L2 Polyclonal (CL488-16480)

    FC experiment of HepG2 using CL488-16480

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human NDUFA4L2 (CL488-16480) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Geprüfte Anwendungen

Erfolgreiche Detektion in FCHepG2-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Durchflusszytometrie (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

CL488-16480 bindet in FC (Intra) NDUFA4L2 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen NDUFA4L2 fusion protein Ag9600
Vollständiger Name NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 4-like 2
Berechnetes Molekulargewicht 87 aa, 10 kDa
Beobachtetes Molekulargewicht 10 kDa
GenBank-ZugangsnummerBC011910
Gene symbol NDUFA4L2
Gene ID (NCBI) 56901
Konjugation CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
LagerungsbedingungenBei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NDUFA4L2(NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 4-like 2), also named as NUOMS, is a HIF-1α target gene that localizes to the mitochondria and belongs to the complex I NDUFA4 subunit family. It downregulates oxygen consumption and Complex I activity in hypoxia. NDUFA4L2 is involved in hypoxic adaptation by decreasing mitochondrial ROS production.

Protokolle

Produktspezifische Protokolle
FC protocol for CL Plus 488 NDUFA4L2 antibody CL488-16480Protokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen