NDUFA5 Rekombinanter Antikörper

NDUFA5 Rekombinant Antikörper für WB, IHC, Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

242860B10

Kat-Nr. : 85187-1-PBS

Synonyme

242860B10, B13, CI 13KD B, CI-13kD-B, Complex I 13kD B

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  WB analysis using 85187-1-RR (same clone as 85187-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85187-1-RR (NDUFA5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85187-1-PBS in a different storage buffer formulation.

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  WB analysis using 85187-1-RR (same clone as 85187-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85187-1-RR (NDUFA5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85187-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 85187-1-RR (same clone as 85187-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 85187-1-RR (NDUFA5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85187-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 85187-1-RR (same clone as 85187-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 85187-1-RR (NDUFA5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85187-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP01899-1

  Cytometric bead array standard curve of MP01899-1, NDUFA5 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85187-1-PBS. Detection antibody: 85187-4-PBS. Standard: Ag9995. Range: 0.781-100 ng/mL.

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  Affinity and Kinetic Characterization of 85187-1-RR

  Biolayer interferometry (BLl) kinetic assays of 85187-1-RR against Human NDUFA5 were performed. The affinity constant is 0.378 nM.

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Geprüfte Anwendungen

Produktinformation

85187-1-PBS bindet in WB, IHC, Cytometric bead array, Indirect ELISA NDUFA5 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: NDUFA5 fusion protein Ag9995
• Vollständiger Name: NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 5, 13kDa
• Berechnetes Molekulargewicht: 13 kDa
• Beobachtetes Molekulargewicht: 13 kDa
• GenBank-Zugangsnummer: BC000813
• Gene symbol: NDUFA5
• Gene ID (NCBI): 4698
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NDUFA5, also named as CI-13kD-B and NDUA5, belongs to the complex I NDUFA5 subunit family. It is accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) which functions in the transfer of electrons from NADH to the respiratory chain. NDUFA5,an enrichment of mitochondrial protein, can be used as a mitochondrial marker protein(PMID:21360670).