NDUFA6 Polyklonaler Antikörper

NDUFA6 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 15445-1-AP

Synonyme

B14, CI B14, Complex I B14, LYR motif containing protein 6, LYRM6, NADHB14, NDUFA6

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  WB analysis using 15445-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15445-1-AP (NDUFA6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 15445-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 15445-1-AP (NDUFA6 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 15445-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 15445-1-AP (NDUFA6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 15445-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15445-1-AP (NDUFA6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 15445-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15445-1-AP (NDUFA6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausherzgewebe, humanes Herzgewebe, Rattenherzgewebe
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

15445-1-AP bindet in WB, IHC, ELISA NDUFA6 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NDUFA6 fusion protein Ag7593
• Vollständiger Name: NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 6, 14kDa
• Berechnetes Molekulargewicht: 15 kDa
• Beobachtetes Molekulargewicht: 15 kDa
• GenBank-Zugangsnummer: BC002772
• Gene symbol: NDUFA6
• Gene ID (NCBI): 4700
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NDUFA6 (NADH:Ubiquinone Oxidoreductase Subunit A6), also called LYR motif-containing protein 6 (LYRM6), or Complex I-B14 (CI-B14), or NADH-ubiquinone oxidoreductase B14 subunit, is an accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I). NDUFA6 participates in the regulation of adipogenic differentiation.