NDUFA9 Polyklonaler Antikörper

NDUFA9 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (2)

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(26)

Kat-Nr. : 20312-1-AP

Synonyme

CI 39kD, CI-39kD, Complex I-39kD, NADH-ubiquinone oxidoreductase 39 kDa subunit, NDUFS2L

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  WB analysis using 20312-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20312-1-AP (NDUFA9 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 20312-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20312-1-AP (NDUFA9 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse liver using 20312-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 20312-1-AP (NDUFA9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 20312-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 20312-1-AP (NDUFA9 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse testis using 20312-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse testis tissue using NDUFA9 antibody (20312-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse testis using 20312-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse testis tissue using NDUFA9 antibody (20312-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse liver using 20312-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using NDUFA9 antibody (20312-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse liver using 20312-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using NDUFA9 antibody (20312-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, Mausherzgewebe, Rattenherzgewebe
• Erfolgreiche Detektion in IHC: Mauslebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 25 publications below
• IHC: See 1 publications below

Produktinformation

20312-1-AP bindet in WB, IHC, ELISA NDUFA9 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus, Zebrafisch
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NDUFA9 fusion protein Ag14150
• Vollständiger Name: NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 9, 39kDa
• Berechnetes Molekulargewicht: 377 aa, 43 kDa
• Beobachtetes Molekulargewicht: 35 kDa
• GenBank-Zugangsnummer: BC009311
• Gene symbol: NDUFA9
• Gene ID (NCBI): 4704
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NDUFA9(NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial) is also named as NDUFS2L, CI-39kD and belongs to the complex I NDUFA9 subunit family. It is involved in stabilizing the junction between membrane and matrix arms of complex I, a late assembly step critical for complex I biogenesis and activity.

Publikationen

Species	Application	Title
human	WB	Mol Cell; Hepatic micropeptide modulates mitochondrial RNA processing machinery in hepatocellular carcinoma; Authors - Linyu Zhu
mouse	WB	Research (Wash D C); LonP1 Links Mitochondria-ER Interaction to Regulate Heart Function; Authors - Yujie Li
human	WB	EMBO J; EglN2 associates with the NRF1-PGC1α complex and controls mitochondrial function in breast cancer.; Authors - Jing Zhang
mouse	WB	Dev Cell; Regulation of reverse electron transfer at mitochondrial complex I by unconventional Notch action in cancer stem cells.; Authors - Rani Ojha
human	WB	J Cell Biol; FAM92A1 is a BAR domain protein required for mitochondrial ultrastructure and function.; Authors - Liang Wang
human	WB	Cell Mol Life Sci; GTPBP8 is required for mitoribosomal biogenesis and mitochondrial translation; Authors - Liang Wang