NDUFAF1 Polyklonaler Antikörper

NDUFAF1 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 15181-1-AP

Synonyme

Complex I intermediate-associated protein 30, mitochondrial, CIA30, CGI-65, CGI65, CGI 65

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  WB analysis of HEK-293 using 15181-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 15181-1-AP (NDUFAF1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 15181-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 15181-1-AP (NDUFAF1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of Transfected HEK-293 using 15181-1-AP

  Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 15181-1-AP (NDUFAF1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 15181-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15181-1-AP (NDUFAF1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human oesophagus cancer using 15181-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 15181-1-AP (NDUFAF1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human oesophagus cancer using 15181-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 15181-1-AP (NDUFAF1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human oesophagus cancer using 15181-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 15181-1-AP (NDUFAF1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, Jurkat-Zellen, Transfizierte HEK-293-Zellen
• Erfolgreiche Detektion in IHC: humanes Ösophaguskarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunhistochemie (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

15181-1-AP bindet in WB, IHC, ELISA NDUFAF1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NDUFAF1 fusion protein Ag7162
• Vollständiger Name: NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, assembly factor 1
• Berechnetes Molekulargewicht: 38 kDa
• Beobachtetes Molekulargewicht: 32 kDa
• GenBank-Zugangsnummer: BC000780
• Gene symbol: NDUFAF1
• Gene ID (NCBI): 51103
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	WB	Stem Cell Res Ther; Mitochondrial activity regulates the differentiation of skin-derived mesenchymal stem cells into brown adipocytes to contribute to hypertension.; Authors - Wenda Xi
mouse	WB	Sci Rep; Integrative analyses of translatome and transcriptome reveal important translational controls in brown and white adipose regulated by microRNAs.; Authors - David W Reid