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NDUFAF3 Polyklonaler Antikörper

NDUFAF3 Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 25621-1-PBS

Synonyme

NDUF3, E3 3, C3orf60, 2P1

Filter:
  • Western Blot (WB) analysis of various lysates using NDUFAF3 Polyclonal antibody (25621-1-AP)

    WB analysis using 25621-1-AP (same clone as 25621-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 25621-1-AP (NDUFAF3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25621-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using NDUFAF3 Polyclonal antibody (25621-1-AP)

    IHC staining of human ovary cancer using 25621-1-AP (same clone as 25621-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 25621-1-AP (NDUFAF3 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25621-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using NDUFAF3 Polyclonal antibody (25621-1-AP)

    IF Staining of HeLa using 25621-1-AP (same clone as 25621-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NDUFAF3 antibody (25621-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 25621-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

25621-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA NDUFAF3 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen NDUFAF3 fusion protein Ag22514
Vollständiger Name NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, assembly factor 3
Berechnetes Molekulargewicht 184 aa, 20 kDa
Beobachtetes Molekulargewicht17 kDa
GenBank-ZugangsnummerBC002873
Gene symbol NDUFAF3
Gene ID (NCBI) 25915
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NDUFAF3 has been assigned to be an assembly factor of the Q-module because it physically associates with Q-module subassemblies during complex I biogenesis (PMID: 27986404).