NDUFB2 Polyklonaler Antikörper

NDUFB2 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 17614-1-AP

Synonyme

NADH-ubiquinone oxidoreductase AGGG subunit, Complex I-AGGG, Complex I AGGG, CI-AGGG, CI AGGG

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  WB analysis of human heart using 17614-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 17614-1-AP (NDUFB2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 17614-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17614-1-AP (NDUFB2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 17614-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 17614-1-AP (NDUFB2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 17614-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 17614-1-AP (NDUFB2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat heart using 17614-1-AP

  rat heart tissue were subjected to SDS PAGE followed by western blot with 17614-1-AP (NDUFB2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse spleen using 17614-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 17614-1-AP (NDUFB2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 17614-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17614-1-AP (NDUFB2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 17614-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17614-1-AP (NDUFB2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human brain using 17614-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 17614-1-AP (NDUFB2 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human brain using 17614-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 17614-1-AP (NDUFB2 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human ovary using 17614-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 17614-1-AP (NDUFB2 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human ovary using 17614-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 17614-1-AP (NDUFB2 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human heart using 17614-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 17614-1-AP (NDUFB2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human heart using 17614-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 17614-1-AP (NDUFB2 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human kidney using 17614-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17614-1-AP (NDUFB2 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human kidney using 17614-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17614-1-AP (NDUFB2 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human skin using 17614-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 17614-1-AP (NDUFB2 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human skin using 17614-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 17614-1-AP (NDUFB2 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human liver using 17614-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 17614-1-AP (NDUFB2 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human liver using 17614-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 17614-1-AP (NDUFB2 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of C2C12 using 17614-1-AP

  Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using NDUFB2 antibody (17614-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293T-Zellen, HuH-7-Zellen, humanes Herzgewebe, MCF-7-Zellen, Maushirngewebe, Mausherzgewebe, Mausnierengewebe, Mausmilzgewebe, Rattenherzgewebe
• Erfolgreiche Detektion in IHC: Maushirngewebe, humanes Hirngewebe, humanes Herzgewebe, humanes Nierengewebe, humanes Lebergewebe, humanes Eierstockgewebe, humanes Hautgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: C2C12-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

17614-1-AP bindet in WB, IHC, IF/ICC, ELISA NDUFB2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NDUFB2 fusion protein Ag11789
• Vollständiger Name: NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 2, 8kDa
• Berechnetes Molekulargewicht: 95 aa, 11 kDa
• Beobachtetes Molekulargewicht: 8-12 kDa
• GenBank-Zugangsnummer: BC063026
• Gene symbol: NDUFB2
• Gene ID (NCBI): 4708
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The multisubunit NADH:ubiquinone oxidoreductase (complex I) is the first enzyme complex in the electron transport chain of mitochondria. NDUFB2, also named as CI-AGGG, is a nuclear encoded subunit located in the hydrophobic protein fraction of complex I(PMID:9878551). It may have a role in the regulation of molecular changes during the global cardiac ischemia/reperfusion injury during cardiopulmonary bypass (CPB). The full length 12 kDa protein has a transit peptide with 33 amino acids.

Publikationen

Species	Application	Title
mouse	WB	Front Immunol; Identification and validation of cuproptosis-related genes in acetaminophen-induced liver injury using bioinformatics analysis and machine learning; Authors - Zhenya Guo