NDUFB7 Monoklonaler Antikörper

NDUFB7 Monoklonal Antikörper für Indirect ELISA, IHC, WB

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Kaninchen, Maus, Ratte

Anwendung

Indirect ELISA, IHC, WB

Konjugation

Unkonjugiert

CloneNo.

1H8C1

Kat-Nr. : 68362-1-PBS

Synonyme

Complex I B18, CI-B18, CI B18, Cell adhesion protein SQM1, B18

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  WB analysis using 68362-1-Ig (same clone as 68362-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68362-1-Ig (NDUFB7 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68362-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 68362-1-Ig (same clone as 68362-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 68362-1-Ig (NDUFB7 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68362-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 68362-1-Ig (same clone as 68362-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 68362-1-Ig (NDUFB7 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68362-1-PBS in a different storage buffer formulation.

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  FC experiment of HEK-293 using 68362-1-Ig (same clone as 68362-1-PBS)

  1X10^6 HEK-293 cells were intracellularly stained with 0.4 ug Anti-Human NDUFB7 (68362-1-Ig, Clone:1H8C1) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 68362-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

68362-1-PBS bindet in Indirect ELISA, IHC, WB NDUFB7 und zeigt Reaktivität mit Hausschwein, human, Kaninchen, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Kaninchen, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: NDUFB7 fusion protein Ag6720
• Vollständiger Name: NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 7, 18kDa
• Berechnetes Molekulargewicht: 16 kDa
• Beobachtetes Molekulargewicht: 18 kDa
• GenBank-Zugangsnummer: BC002595
• Gene symbol: NDUFB7
• Gene ID (NCBI): 4713
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NDUFB7(NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7) is also named as CI-B18 and belongs to the complex I NDUFB7 subunit family. It is an accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), which couples the oxidation of NADH to the reduction of ubiquinone and the translocation of protons across the inner membrane. NDUFB7 protein lacks a mitochondrial targeting signal and transmembrane domains. However, it has a Cx(9)C motif that includes an intermembrane space targeting signal.(PMID:21310150).