NDUFB8 Polyklonaler Antikörper

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• KD/KO Validated

NDUFB8 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (2)

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

37

Rezensionen (1)

Kat-Nr. : 14794-1-AP

Synonyme

ASHI, CI ASHI, Complex I ASHI, NDUFB8

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Galerie der Validierungsdaten

• 

  WB analysis using 14794-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14794-1-AP (NDUFB8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of various samples using 14794-1-AP

  These five antibodies, ATP5A1 (14676-1-AP, 1:8000), UQCRC2 (14742-1-AP, 1:4000), SDHB (10620-1-AP, 1:8000), MTCO2 (55070-1-AP, 1:1000), NDUFB8 (14794-1-AP, 1:5000), can be assembled OXPHOS kit to detect the relative levels of the 5 OXPHOS complexes in mitochondrial preparations.

• 

  WB analysis of A549 using 14794-1-AP

  WB result of NDUFB8 antibody (14794-1-AP; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NDUFB8 transfected A549 cells.

• 

  IP experiment of HepG2 using 14794-1-AP

  IP result of anti-NDUFB8(IP:14794-1-AP, 4ug; Detection:14794-1-AP 1:2000) with HepG2 cells lysate 880 ug.

• 

  IHC staining of human liver using 14794-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14794-1-AP (NDUFB8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 14794-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14794-1-AP (NDUFB8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human heart using 14794-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 14794-1-AP (NDUFB8 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 14794-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 14794-1-AP (NDUFB8 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 14794-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NDUFB8 antibody (14794-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, 3T3-L1-Zellen, A549-Zellen, Maushirngewebe, Mauslebergewebe, Rattenhirngewebe
• Erfolgreiche IP: HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Lebergewebe, humanes Herzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 34 publications below
• IHC: See 2 publications below
• IF: See 3 publications below

Produktinformation

14794-1-AP bindet in WB, IP, IHC, IF, ELISA NDUFB8 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus, Ratte, Zebrafisch
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NDUFB8 fusion protein Ag6495
• Vollständiger Name: NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 8, 19kDa
• Berechnetes Molekulargewicht: 22 kDa
• Beobachtetes Molekulargewicht: 19-22 kDa
• GenBank-Zugangsnummer: BC000466
• Gene symbol: NDUFB8
• Gene ID (NCBI): 4714
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	WB	Sci Adv; Mitochondrial proteostasis stress in muscle drives a long-range protective response to alleviate dietary obesity independently of ATF4.; Authors - Qiqi Guo
mouse	WB	Nat Commun; Disuse-associated loss of the protease LONP1 in muscle impairs mitochondrial function and causes reduced skeletal muscle mass and strength.; Authors - Zhisheng Xu
mouse	WB	J Exp Med; FNIP1 regulates adipocyte browning and systemic glucose homeostasis in mice by shaping intracellular calcium dynamics.; Authors - Yujing Yin
mouse	WB	Biomaterials; Role of molybdenum in material immunomodulation and periodontal wound healing: Targeting immunometabolism and mitochondrial function for macrophage modulation.; Authors - Xiao-Tao He
pig	WB	J Cachexia Sarcopenia Muscle; MyD88 deficiency ameliorates weight loss caused by intestinal oxidative injury in an autophagy-dependent mechanism.; Authors - Ming Qi
mouse	WB	Cell Rep; Mitophagy Directs Muscle-Adipose Crosstalk to Alleviate Dietary Obesity.; Authors - Tingting Fu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Edward (Verified Customer) (11-15-2022)

Used with 12 ug protein lysate NDUFB8 works well, both with ECL detection and fluorescent secondary antibodies it gives a strong band

• Applications: Western Blot
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: K562 and KCL22