NDUFC2 Polyklonaler Antikörper

NDUFC2 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

Kat-Nr. : 15573-1-AP

Synonyme

HLC 1, Complex I-B14.5b, Complex I B14.5b, CI-B14.5b, CI B14.5b

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  WB analysis using 15573-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15573-1-AP (NDUFC2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 15573-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15573-1-AP (NDUFC2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 15573-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 15573-1-AP (NDUFC2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human kidney using 15573-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 15573-1-AP (NDUFC2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 15573-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15573-1-AP (NDUFC2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 15573-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15573-1-AP (NDUFC2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human hepatocirrhosis using 15573-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 15573-1-AP (NDUFC2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human hepatocirrhosis using 15573-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 15573-1-AP (NDUFC2 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human kidney using 15573-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15573-1-AP (NDUFC2 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human kidney using 15573-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15573-1-AP (NDUFC2 antibody) at dilution of 1:200 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, HeLa-Zellen, HepG2-Zellen, humanes Nierengewebe, humanes Lebergewebe, Maushirngewebe, Mausherzgewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: humanes Nierengewebe, humanes Leberzirrhosegewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below

Produktinformation

15573-1-AP bindet in WB, IHC, ELISA NDUFC2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NDUFC2 fusion protein Ag7919
• Vollständiger Name: NADH dehydrogenase (ubiquinone) 1, subcomplex unknown, 2, 14.5kDa
• Berechnetes Molekulargewicht: 14 kDa
• Beobachtetes Molekulargewicht: 14 kDa
• GenBank-Zugangsnummer: BC007323
• Gene symbol: NDUFC2
• Gene ID (NCBI): 4718
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	WB	Nat Commun; Intellectual disability-associated factor Zbtb11 cooperates with NRF-2/GABP to control mitochondrial function.; Authors - Brooke C Wilson
pig	WB	Sci Rep; Proteomic analysis indicates that mitochondrial energy metabolism in skeletal muscle tissue is negatively correlated with feed efficiency in pigs.; Authors - Liangliang Fu
human	WB	Cell Rep; A membrane arm of mitochondrial complex I sufficient to promote respirasome formation.; Authors - Hezhi Fang
mouse	WB	Environ Int; Bisphenol S impairs mitochondrial function by targeting Myo19/oxidative phosphorylation pathway contributing to axonal and dendritic injury; Authors - Xing Zhang