NDUFS1 Polyklonaler Antikörper

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• KD/KO Validated

NDUFS1 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 12444-1-PBS

Synonyme

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  WB analysis using 12444-1-AP (same clone as 12444-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 12444-1-AP (NDUFS1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12444-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 12444-1-AP (same clone as 12444-1-PBS)

  WB result of NDUFS1 antibody (12444-1-AP; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NDUFS1 transfected HEK-293 cells. This data was developed using the same antibody clone with 12444-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 12444-1-AP (same clone as 12444-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 12444-1-AP (NDUFS1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12444-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 12444-1-AP (same clone as 12444-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 12444-1-AP (NDUFS1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12444-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse liver using 12444-1-AP (same clone as 12444-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 12444-1-AP (NDUFS1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12444-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A549 using 12444-1-PBS

  A549 cells were subjected to SDS PAGE followed by western blot with 12444-1-AP (NDUFS1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 12444-1-AP (same clone as 12444-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 12444-1-AP (NDUFS1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12444-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A549 using 12444-1-AP (same clone as 12444-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 12444-1-AP (NDUFS1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12444-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse liver using 12444-1-PBS

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 12444-1-AP (NDUFS1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse lung using 12444-1-AP (same clone as 12444-1-PBS)

  IP result of anti-NDUFS1 (IP:12444-1-AP, 4ug; Detection:12444-1-AP 1:500) with mouse lung tissue lysate 2800ug. This data was developed using the same antibody clone with 12444-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 12444-1-AP (same clone as 12444-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12444-1-AP (NDUFS1 antibody) at dilution of 1:200 (under 40x lens) Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0). This data was developed using the same antibody clone with 12444-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 12444-1-AP (same clone as 12444-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12444-1-AP (NDUFS1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12444-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 12444-1-AP (same clone as 12444-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12444-1-AP (NDUFS1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12444-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 12444-1-AP (same clone as 12444-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12444-1-AP (NDUFS1 antibody) at dilution of 1:200 (under 10x lens) Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0). This data was developed using the same antibody clone with 12444-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 12444-1-AP (same clone as 12444-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NDUFS1 antibody (12444-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 12444-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 12444-1-AP (same clone as 12444-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NDUFS1 antibody (12444-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 12444-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

12444-1-PBS bindet in WB, IHC, IF/ICC, IP, ELISA NDUFS1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NDUFS1 fusion protein Ag3135
• Vollständiger Name: NADH dehydrogenase (ubiquinone) Fe-S protein 1, 75kDa (NADH-coenzyme Q reductase)
• Berechnetes Molekulargewicht: 727 aa, 79 kDa
• Beobachtetes Molekulargewicht: 74-81 kDa
• GenBank-Zugangsnummer: BC030833
• Gene symbol: NDUFS1
• Gene ID (NCBI): 4719
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The multisubunit NADH:ubiquinone oxidoreductase (75 kDa subunit, mitochondrial)(NDUFS1) is the first enzyme complex in the electron transport chain of mitochondria. It is also named as Complex I-75kD. By use of chaotropic agents, complex I can be fragmented into 3 different fractions: a flavoprotein fraction, an iron-sulfur protein (IP) fraction, and a hydrophobic protein (HP) fraction. NDUFS1 is the largest subunit of complex I and it is a component of the iron-sulfur (IP) fragment of the enzyme. NDUFS1 has some isoforms with the molecular mass of 68-81 kDa.