NDUFS2 Rekombinanter Antikörper

NDUFS2 Rekombinant Antikörper für WB, IHC, FC (Intra), Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, FC (Intra), Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

242238D4

Kat-Nr. : 84880-1-PBS

Synonyme

242238D4, CI 49kD, CI-49kD, Complex I 49kD, Complex I-49kD

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  WB analysis using 84880-1-RR (same clone as 84880-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84880-1-RR (NDUFS2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84880-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 84880-1-RR (same clone as 84880-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 84880-1-RR (NDUFS2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84880-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 84880-1-RR (same clone as 84880-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 84880-1-RR (NDUFS2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84880-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 84880-1-RR (same clone as 84880-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84880-1-RR (NDUFS2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84880-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse liver using 84880-1-RR (same clone as 84880-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84880-1-RR (NDUFS2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84880-1-PBS in a different storage buffer formulation.

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  FC experiment of HepG2 using 84880-1-RR (same clone as 84880-1-PBS)

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug NDUFS2 Recombinant antibody (84880-1-RR, Clone:242238D4) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84880-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 84880-1-RR (same clone as 84880-1-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug NDUFS2 Recombinant antibody (84880-1-RR, Clone:242238D4) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84880-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP01653-2

  Cytometric bead array standard curve of MP01653-2, NDUFS2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84880-3-PBS. Detection antibody: 84880-1-PBS. Standard: Ag28008. Range: 0.938-60 ng/mL.

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  Affinity and Kinetic Characterization of 84880-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84880-1-RR against Human NDUFS2 were performed. The affinity constant is 1.61 nM.

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Geprüfte Anwendungen

Produktinformation

84880-1-PBS bindet in WB, IHC, FC (Intra), Cytometric bead array, Indirect ELISA NDUFS2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: NDUFS2 fusion protein Ag28008
• Vollständiger Name: NADH dehydrogenase (ubiquinone) Fe-S protein 2, 49kDa (NADH-coenzyme Q reductase)
• Berechnetes Molekulargewicht: 53 kDa
• Beobachtetes Molekulargewicht: 40-50 kDa
• GenBank-Zugangsnummer: BC000170
• Gene symbol: NDUFS2
• Gene ID (NCBI): 4720
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Mitochondrial Complex I, responsible for the oxidation of NADH, is suspected to be an integral component of the O2-sensing pathway. NDUFS2 (NADH:Ubiquinone Oxidoreductase Core Subunit S2), a key subunit of complex I in the mitochondrial electron transport chain (ETC), is essential for acute oxygen-sensing and hypoxic pulmonary vasoconstriction (PMID: 30922174). Functions of NDUFS2 are associated with growth, cell-membrane integrity, ROS generation, apoptosis, necrosis, Complex I respiration, and synthesis of ATP (PMID: 33744462).