NDUFS3 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

NDUFS3 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

29

Kat-Nr. : 15066-1-AP

Synonyme

CI 30kD, Complex I 30kD, NDUFS3

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Galerie der Validierungsdaten

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  WB analysis using 15066-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15066-1-AP (NDUFS3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 15066-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15066-1-AP (NDUFS3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse heart using 15066-1-AP

  IP Result of anti-NDUFS3 (IP:15066-1-AP, 4ug; Detection:15066-1-AP 1:500) with mouse heart tissue lysate 4000ug.

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  IHC staining of mouse testis using 15066-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15066-1-AP (NDUFS3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 15066-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using NDUFS3 antibody (15066-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293T-Zellen, HepG2-Zellen, Mausherzgewebe, Maushirngewebe, NIH/3T3-Zellen, Rattenherzgewebe, Rattenhirngewebe
• Erfolgreiche IP: Mausherzgewebe
• Erfolgreiche Detektion in IHC: Maushodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 25 publications below
• IHC: See 5 publications below
• IF: See 1 publications below

Produktinformation

15066-1-AP bindet in WB, IP, IHC, IF, ELISA NDUFS3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NDUFS3 fusion protein Ag7072
• Vollständiger Name: NADH dehydrogenase (ubiquinone) Fe-S protein 3, 30kDa (NADH-coenzyme Q reductase)
• Berechnetes Molekulargewicht: 30 kDa
• Beobachtetes Molekulargewicht: 30 kDa
• GenBank-Zugangsnummer: BC000617
• Gene symbol: NDUFS3
• Gene ID (NCBI): 4722
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NDUFS3(NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial) is also named as CI-30kD and belongs to the complex I 30 kDa subunit family. It catalyzes the oxidation of NADH, with the concomitant reduction of ubiquinone and ejection of protons out of the mitochondria(PMID:10967146). NDUFS3 can be used as a mitochondrial matrix control(PMID:17344420). The full length has a transit peptide with 36 amino acids.

Publikationen

Species	Application	Title
human	WB,IHC	Cancer Discov; NCOA4-Mediated Ferritinophagy Is a Pancreatic Cancer Dependency via Maintenance of Iron Bioavailability for Iron-Sulfur Cluster Proteins; Authors - Naiara Santana-Codina
human	WB	Mol Cell; Maintaining Iron Homeostasis Is the Key Role of Lysosomal Acidity for Cell Proliferation.; Authors - Ross A Weber
human	WB	EMBO Rep; AIF inhibits tumor metastasis by protecting PTEN from oxidation.; Authors - Shao-Ming Shen
human	WB	J Cell Biol; PDGFRβ translocates to the nucleus and regulates chromatin remodeling via TATA element-modifying factor 1.; Authors - Natalia Papadopoulos
mouse	WB	Diabetes; DW14006 as a Direct AMPKα Activator Ameliorates Diabetic Peripheral Neuropathy in Mice.; Authors - Xu Xu
human	WB	J Med Genet; COA7 (C1orf163/RESA1) mutations associated with mitochondrial leukoencephalopathy and cytochrome c oxidase deficiency.; Authors - Anabel Martinez Lyons