NDUFS3 Polyklonaler Antikörper

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• KD/KO Validated

NDUFS3 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 15066-1-PBS

Synonyme

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  WB analysis using 15066-1-AP (same clone as 15066-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 15066-1-AP (NDUFS3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15066-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 15066-1-AP (same clone as 15066-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 15066-1-AP (NDUFS3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15066-1-PBS in a different storage buffer formulation.

• 

  IP experiment of mouse heart using 15066-1-AP (same clone as 15066-1-PBS)

  IP result of anti-NDUFS3 (IP:15066-1-AP, 4ug; Detection:15066-1-AP 1:500) with mouse heart tissue lysate 4000ug. This data was developed using the same antibody clone with 15066-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 15066-1-AP (same clone as 15066-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15066-1-AP (NDUFS3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15066-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 15066-1-AP (same clone as 15066-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using NDUFS3 antibody (15066-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 15066-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

15066-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA NDUFS3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NDUFS3 fusion protein Ag7072
• Vollständiger Name: NADH dehydrogenase (ubiquinone) Fe-S protein 3, 30kDa (NADH-coenzyme Q reductase)
• Berechnetes Molekulargewicht: 30 kDa
• Beobachtetes Molekulargewicht: 30 kDa
• GenBank-Zugangsnummer: BC000617
• Gene symbol: NDUFS3
• Gene ID (NCBI): 4722
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NDUFS3(NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial) is also named as CI-30kD and belongs to the complex I 30 kDa subunit family. It catalyzes the oxidation of NADH, with the concomitant reduction of ubiquinone and ejection of protons out of the mitochondria(PMID:10967146). NDUFS3 can be used as a mitochondrial matrix control(PMID:17344420). The full length has a transit peptide with 36 amino acids.