NDUFS3 Monoklonaler Antikörper

NDUFS3 Monoklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Kaninchen, Maus, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1A1B3

Kat-Nr. : 68066-1-PBS

Synonyme

CI 30kD, Complex I 30kD, NDUFS3

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  WB analysis using 68066-1-Ig (same clone as 68066-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68066-1-Ig (NDUFS3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 68066-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig brain using 68066-1-Ig (same clone as 68066-1-PBS)

  pig brain tissue were subjected to SDS PAGE followed by western blot with 68066-1-Ig (NDUFS3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68066-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rabbit brain using 68066-1-Ig (same clone as 68066-1-PBS)

  rabbit brain tissue were subjected to SDS PAGE followed by western blot with 68066-1-Ig (NDUFS3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68066-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat brain using 68066-1-Ig (same clone as 68066-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 68066-1-Ig (NDUFS3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68066-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse brain using 68066-1-Ig (same clone as 68066-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 68066-1-Ig (NDUFS3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68066-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 68066-1-Ig (same clone as 68066-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 68066-1-Ig (NDUFS3 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68066-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 68066-1-Ig (same clone as 68066-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 68066-1-Ig (NDUFS3 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68066-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

68066-1-PBS bindet in WB, IHC, Indirect ELISA NDUFS3 und zeigt Reaktivität mit Hausschwein, human, Kaninchen, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Kaninchen, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: NDUFS3 fusion protein Ag7072
• Vollständiger Name: NADH dehydrogenase (ubiquinone) Fe-S protein 3, 30kDa (NADH-coenzyme Q reductase)
• Berechnetes Molekulargewicht: 30 kDa
• Beobachtetes Molekulargewicht: 30 kDa
• GenBank-Zugangsnummer: BC000617
• Gene symbol: NDUFS3
• Gene ID (NCBI): 4722
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NDUFS3(NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial) is also named as CI-30kD and belongs to the complex I 30 kDa subunit family. It catalyzes the oxidation of NADH, with the concomitant reduction of ubiquinone and ejection of protons out of the mitochondria(PMID:10967146). NDUFS3 can be used as a mitochondrial matrix control(PMID:17344420). The full length has a transit peptide with 36 amino acids.