NDUFS3 Rekombinanter Antikörper

NDUFS3 Rekombinant Antikörper für WB, IF/ICC, Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

251654E6

Kat-Nr. : 86676-1-PBS

Synonyme

CI 30kD, CI30kD, CI-30kD, Complex I-30kD, EC:7.1.1.2

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  WB analysis using 86676-1-RR (same clone as 86676-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86676-1-RR (NDUFS3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86676-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 86676-1-RR (same clone as 86676-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NDUFS3 antibody (86676-1-RR, Clone: 251654E6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86676-1-PBS in a different storage buffer formulation.

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  Cytometric bead array sample test of MP02593-1

  The mean NDUFS3 concentration was determined to be 89.8 ng/mL in HEK-293T cell extract based on a 1.3 mg/mL extract load, 74.3 ng/mL in HepG2 cell extract based on a 1.2 mg/mL extract load.

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  Cytometric bead array standard curve of MP02593-1

  Cytometric bead array standard curve of MP02593-1, NDUFS3 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 86676-2-PBS. Detection antibody: 86676-1-PBS. Standard: Ag7072. Range: 0.781-100 ng/mL.

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  Affinity and Kinetic Characterization of 86676-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86676-1-RR against Human NDUFS3 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

Produktinformation

86676-1-PBS bindet in WB, IF/ICC, Cytometric bead array, Indirect ELISA NDUFS3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: NDUFS3 fusion protein Ag7072
• Vollständiger Name: NADH dehydrogenase (ubiquinone) Fe-S protein 3, 30kDa (NADH-coenzyme Q reductase)
• Berechnetes Molekulargewicht: 30 kDa
• Beobachtetes Molekulargewicht: 30 kDa
• GenBank-Zugangsnummer: BC000617
• Gene symbol: NDUFS3
• Gene ID (NCBI): 4722
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NDUFS3(NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial) is also named as CI-30kD and belongs to the complex I 30 kDa subunit family. It catalyzes the oxidation of NADH, with the concomitant reduction of ubiquinone and ejection of protons out of the mitochondria(PMID:10967146). NDUFS3 can be used as a mitochondrial matrix control(PMID:17344420).