NDUFS4 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

NDUFS4 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 15849-1-AP

Synonyme

AQDQ, CI 18 kDa, CI AQDQ, Complex I 18 kDa, Complex I AQDQ, NDUFS4

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Galerie der Validierungsdaten

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  WB analysis of mouse heart using 15849-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 15849-1-AP (NDUFS4 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of rat heart using 15849-1-AP

  rat heart tissue were subjected to SDS PAGE followed by western blot with 15849-1-AP (NDUFS4 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 15849-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 15849-1-AP (NDUFS4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon using 15849-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 15849-1-AP (NDUFS4 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human colon using 15849-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 15849-1-AP (NDUFS4 Antibody) at dilution of 1:200 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausherzgewebe, Rattenherzgewebe
• Erfolgreiche Detektion in IHC: humanes Kolongewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunhistochemie (IHC): IHC : 1:100-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 4 publications below

Produktinformation

15849-1-AP bindet in WB, IHC, ELISA NDUFS4 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NDUFS4 fusion protein Ag8633
• Vollständiger Name: NADH dehydrogenase (ubiquinone) Fe-S protein 4, 18kDa (NADH-coenzyme Q reductase)
• Berechnetes Molekulargewicht: 175 aa, 20 kDa
• Beobachtetes Molekulargewicht: 21 kDa
• GenBank-Zugangsnummer: BC005270
• Gene symbol: NDUFS4
• Gene ID (NCBI): 4724
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NDUFS4 (NADH dehydrogenase [ubiquinone] iron-sulfur protein 4 mitochondrial), also known as AQDQ, is a member of NADH dehydrogenase (ubiquinone) iron-sulfur (NDUFS) protein family. It is a peripheral membrane protein located on the matrix side of the inner mitochondrial membrane. It is reported that the loss of NDUFS4 protein expression in immunohistochemistry (IHC) reliably diagnoses papillary thyroid carcinoma(PMID: 34792302). Also, NDUFS4 promotes tumor progression and predicts prognosis in gastric cancer(PMID: 36044738).

Publikationen

Species	Application	Title
mouse	WB	Immunity; Metformin inhibition of mitochondrial ATP and DNA synthesis abrogates NLRP3 inflammasome activation and pulmonary inflammation.; Authors - Hongxu Xian ; KO Validated
mouse	WB	Theranostics; Proteomic analysis reveals ginsenoside Rb1 attenuates myocardial ischemia/reperfusion injury through inhibiting ROS production from mitochondrial complex I.; Authors - Lujing Jiang ; KD Validated
mouse	WB	EBioMedicine; SIRT5 deacylates metabolism-related proteins and attenuates hepatic steatosis in ob/ob mice.; Authors - Yipeng Du
rat	WB	Arch Biochem Biophys; Epigallocatechin-3-gallate exerts cardioprotective effects related to energy metabolism in pressure overload-induced cardiac dysfunction.; Authors - Qiuhong Mou